Objective: To investigate the protective effect of hyperoside on H2O2-induced oxidative damage of A549 cells. Method: In vitro oxidative stress damage model was established by treating A549 cells with H2O2 (400 μmol·L-1) for 24 h. Cells were divided into blank group

model group (400 μmol·L-1 H2O2)

positive drug group (N-acetyl cysteine 100 μmol·L-1)

hyperoside groups (1

10

100 μmol·L-1). Cell viability was determined by thiazolyl blue(MTT) assay. Contents of reactive oxygen(ROS) and malondialdehyde(MDA)

lactic dehydrogenase(LDH)

catalase(CAT)

glutathion peroxidase(GSH-Px) and superoxide dismutase(SOD) activity were detected by enzyme-linked immunosorbent assay(ELISA) method. The membrane potential changes of mitochondria were detected by Rhodamine 123 staining

and the protein expression levels of sirtuin 3(Sirt3)

isocitrate dehydrogenase 2(IDH2) and MnSOD were determined by Western blot technology. Result: As compared with model group

hyperoside could significantly enhance the cell viability (P<0.01)

decrease LDH activity

contents of ROS and MDA

and increase GSH-Px and SOD activities (P<0.05

P<0.01). Moreover

hyperoside markedly improved mitochondrial membrane potential

and Western blot showed that hyperoside could significantly up-regulate the protein expression levels of Sirt3

IDH2 and MnSOD (P<0.05

P<0.01). Conclusion: Hyperoside could protect H2O2-induced oxidative damage of A549 cells

and its protective effects may be related with increase in ROS removal ability and regulation of Sirt3 mitochondrial pathway.