Objective: To study on distribution of relative molecular mass of Asini Corii Colla hydrolyzate and discuss its blood enrichment and leukogenic effect. Method: TSK-GEL G2000SWXL column (7.8 mm×300 mm) was employed

mobile phase was consisted of 0.1 mol·L-1 NaCl+0.05% NaN3+0.1 mol·L-1 phosphate buffer solution (pH 6.7) and detection wavelength was set at 280 nm;distributions of relative molecular mass of Asini Corii Colla concentrate

bionic hydrolysate and trypsin hydrolysate were determined.The bloodletting method was adopted to establish blood deficiency mice model

leukopenia model induced by cyclophosphamide in mice were used

blood enrichment and leukogenic effect of Asini Corii Colla hydrolyzate was observed. Result: Relative molecular mass of protein and polypeptide in Asini Corii Colla was greater than 6.64×104 Da

relative molecular mass of bionic hydrolysate and trypsin hydrolysate of Asini Corii Colla mainly concentrated in about 3.7×103 Da.Bionic hydrolysate and trypsin hydrolysate of Asini Corii Colla could significantly improve hemoglobin (Hb) andred cell count (RBC)of blood deficiency model mice

and they could significantly increase white blood cell count (WBC)

RBC and Hb levels of leukopenia model mice. Conclusion: After the enzymolysis

relative molecular mass of Asini Corii Colla decreases and its blood enrichment and leukogenic effect is significant

effect of bionic hydrolysate and trypsin hydrolysate is roughly equivalent.