Objective: To establish the quality evaluation method for Arnebiae Radix based on using DNA barcoding technique

and quantification methods for determination of the active components by HPLC

in order to evaluate the quality of Arnebiae Radix. Method: To identify comfreys collected from different resources by DNA barcoding technique

and analyze the naphthoquinones of Arnebiae Radix by chromatography method. Thermo BDS Hypersil C18 column was used with Acetonitrile-pure water-0.2%acetic acid as mobile phase in gradients elution. The flow rate was 0.8 mL·min-1. The UV detector was monitored at 275 nm

and the column temperature was 35℃. Result: The 11 herbs of different resources were identified as Arnebiae Radix by DNA barcoding techniques. The active components from Arnebiae Radix were analyzed by HPLC chromatography. The components were separated completely under chromatography

and the average recoveries were from 95.26% to 101.40%

its RSD values are between 0.7% and 1.8%(n=6). Conclusion: The methods of molecular identification and HPLC chromatography were combined to evaluate the quality of medicinal materials of shikonin

which are simple and easy to operate. It can be used as quality control methods for shikonin.