Objective: To simultaneously determine the contents of ten flavonoids (genistin

trifolirhizin

maackiain

isoxanthohunol

kushenolI

sophoflavescenol

kurarinone

norkurarinone

2-O-methyl-kurarinone and isokurarinone) by using HPLC method

compare the flavonoid contents in cultivated and wild Sophorae Flavescentis Radix. Method: The chromatographic separation was performed on Kromasil C18 (4.6 mm×250 mm

5 μm) with methanol (A)-0.3% formic acid solution (B) as the mobile phase for gradient elution (0-5 min

50%A; 5-25 min

50%-70%A; 25-35 min

70%A; 35-50 min

70%-90%A; 50-55 min

90%A). The flow rate was 0.8 mL·min-1 and the detection wavelength was set at 295 nm. Independent t-test (t-test)

hierarchical clustering analysis(HCA) and principal components analysis (PCA) were applied to analyze and evaluate the cultivated and the wild Sophorae Flavescentis Radix. Result: The t-test results showed that P>0.05 for all flavonoids

indicating that there were no significant differences in the contents of flavonoids between cultivated and wild Sophorae Flavescentis Radix. HCA and PCA also indicated that there were no differences in the contents of flavonoids between cultivated and wild Sophorae Flavescentis Radix. The results showed that ten flavonoids were well separated and had a good linear relationship

with r>0.999.The average recovery rate was between 95.0%-105.0%

with RSD<5%

consistent with the content determination requirement. Conclusion: The method is accurate and selective

which could provide reference for the quality control of Sophorae Flavescentis Radix.