HE Yao, WANG Li-juan, LIU Ting-ting, et al. Analysis of Therapeutic Mechanism of Siwutang in Primary Dysmenorrhea Syndrome Based on Metabolomics Technique[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(12): 82-89.
DOI:
HE Yao, WANG Li-juan, LIU Ting-ting, et al. Analysis of Therapeutic Mechanism of Siwutang in Primary Dysmenorrhea Syndrome Based on Metabolomics Technique[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(12): 82-89. DOI: 10.13422/j.cnki.syfjx.2017120082.
Analysis of Therapeutic Mechanism of Siwutang in Primary Dysmenorrhea Syndrome Based on Metabolomics Technique
Objective: To study on the mechanism of Siwutang in treating primary dysmenorrhea syndrome from the aspect of endogenous metabolites. Method: Dysmenorrhea rat model was constructed by injecting estradiol benzoate and oxytocin.UPLC-Q-TOF-MS was employed to analyze fingerprint of metabolites in plasma of dysmenorrhea rats under electrospray ionization positive and negative ion mode
the acquired metabolites were identified by mass spectrum informations and public database retrieval.Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were adopted to screen the differential metabolites among the normal group
dysmenorrhea model group and Siwutang group;these different metabolites among three groups were analyzed by KEGG metabolic pathway method. Result: Twenty-three endogenous metabolites from dysmenorrhea model rats were detected and identified;when the dysmenorrhea model group compared with the normal group
progesterone
cortexolone
tauroursodeoxycholic acid and lysophosphatidic acid were the different metabolites with variable importance in the projection (VIP)>1 and P<0.05.When the Siwutang group compared with the model group
L-phenylalanine
L-tyrosine
N-benzoylglycine
L-methionine
cortexolone and stearic acid glycinamide were the different metabolites with VIP>1 and P<0.05. Conclusion: Siwutang has a certain callback effect on the differential metabolites in primary dysmenorrhea rats
its therapeutic mechanism is mainly related to steroid synthesis
lysophosphatidic acid metabolism and amino acid metabolism.
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Related Author
Qing ZHANG
Qiu-yue LUO
Jing LI
Xu-feng PU
XUE Shu-juan
CHEN Sui-qing
ZHANG Jian-shuang
YU Hao
Related Institution
College Pharmacy, Chengdu University of Traditional Chinese Medicine
Chengdu Institute for Food and Drug Control
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