Effect of Bushen Huatan Yizhi Decoction on Learning and Memory Ability and Tau Protein Hosphorylation of Hippocampal Neurons in APPV717I Mice

KONG Ming-wang; ZHAO Jian-bang; SHI He-yuan; LIU Ping; WANG Ping

doi:10.13422/j.cnki.syfjx.2017120096

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Effect of Bushen Huatan Yizhi Decoction on Learning and Memory Ability and Tau Protein Hosphorylation of Hippocampal Neurons in APPV717I Mice

更新时间：2024-01-04

- Effect of Bushen Huatan Yizhi Decoction on Learning and Memory Ability and Tau Protein Hosphorylation of Hippocampal Neurons in APPV717I Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 12, Pages: 96-102(2017)
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- DOI：10.13422/j.cnki.syfjx.2017120096
  CLC： R285.5
- Published：2017
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KONG Ming-wang, ZHAO Jian-bang, SHI He-yuan, et al. Effect of Bushen Huatan Yizhi Decoction on Learning and Memory Ability and Tau Protein Hosphorylation of Hippocampal Neurons in APPV717I Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(12): 96-102.
DOI：

KONG Ming-wang, ZHAO Jian-bang, SHI He-yuan, et al. Effect of Bushen Huatan Yizhi Decoction on Learning and Memory Ability and Tau Protein Hosphorylation of Hippocampal Neurons in APPV717I Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(12): 96-102. DOI： 10.13422/j.cnki.syfjx.2017120096.

摘要

目的：观察补肾化痰益智方（BSHT）对APPV717I转基因小鼠干预效果，揭示其可能作用机制。方法：将3月龄APPV717I转基因小鼠120只分为2批，每批60只，每批又随机分为5组，分别为多奈哌齐（0.000 92 g·kg-1·d-1）组，补肾化痰益智方低、中、高剂量组（5.2，10.4，20.8 g·kg-1·d-1），模型组（APP），每组12只（雌雄各半）；同月龄遗传背景相同的C57BL/6J小鼠24只作为正常组，灌胃给药，模型组及正常组予等容积双蒸水灌胃，每日1次。第1批小鼠给药8个月，第2批给药4个月，最后同时以Morris水迷宫进行行为学检测，分别以酶联免疫吸附测定（ELISA）和免疫印迹法测定（Western blot）海马神经元Tau蛋白磷酸化（p-Ser 199/202，Tau-1）的表达水平。结果：行为学实验结果表明，与正常组比较，模型组小鼠的潜伏期有所延长（P<0.05，P<0.01）；各治疗组与模型组比潜伏期明显缩短（P<0.05）。ELISA和Western blot实验结果表明，与正常组比较，模型组小鼠海马神经元的Ser199/202磷酸化位点磷酸化程度显著升高（P<0.01）；各治疗组Ser199/202磷酸化位点磷酸化水平较模型组比明显缩短（P<0.05，P<0.01）。结论：补肾化痰益智方可以改善Ser199/202磷酸化位点磷酸化程度，防止神经原纤维缠结（NFT）的形成，从而改善APPV717I转基因小鼠早期的学习记忆能力。

Abstract

Objective: To investigate the intervention effect of Bushen Huatan Yizhi decoction(BSHT) in APPV717I transgenic mice and explore its possible mechanism. Method: The 120 three months old APPV717I transgenic mice were randomly divided into two batches

n=60 in each batch. The mice in each batch were randomly divided into model group (APP)

Donepezil group (0.000 92 g·kg-1·d-1)

BSHT low dose group(5.2 g·kg-1·d-1)

middle dose group(10.4 g·kg-1·d-1)

and high dose group(20.8 g·kg-1·d-1)

n=12 in each group (6 males and 6 females). Another 24 C57BL/6J mice of the same age were set as a normal group

and the mice in model group and normal group received intragastric administration of equivalent volume of distilled water

1 time/day. The first batch of mice received drug administration for 8 months

and the second batch for 4 months. Behavioral ability was measured in Morris water maze finally

and the expression levels of Tau protein hosphorylation (p-Ser 199/202

Tau-1) in the hippocampus was determined by using enzyme linked immunosorbent assay(ELISA) and Western blot. Result: Behavioral experiment results showed that

as compared with the normal group

the latency was prolonged in model group mice(P<0.05

P<0.01). After months treatment

the latency was shortened in various treatment groups as compared with model group(P<0.05). ELISA and Western blot assay showed that as compared with the normal group

the phosphorylation degree of Ser199/202 phosphorylation sites in hippocampal neurons was increased significantly in model group(P<0.01); and the phosphorylation degree of Ser199/202 phosphorylation sites in various treatment groups was significantly lower than that in model group(P<0.05

P<0.01). Conclusion: Bushen Huatan Yizhi decoction can improve the phosphorylation degree of Ser199/202 phosphorylation sites

prevent neurofibrillary tangle(NFT) formation

and thus improve the early learning and memory abilities in APPV717I transgenic mice.

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