Serum Containing  Pericarp Inhibits Proliferation and Induces Apoptosis of Gastric Carcinoma SGC-7901 Cell

GAO Qi-long; ZHU Ya-nan; SHI Bian; MA Xu-hui; GENG Liang; ZHANG Xin-feng; NING Han-bing; LIU Huai-min

doi:10.13422/j.cnki.syfjx.2017130111

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Serum Containing Pericarp Inhibits Proliferation and Induces Apoptosis of Gastric Carcinoma SGC-7901 Cell

更新时间：2024-01-04

- Serum Containing Pericarp Inhibits Proliferation and Induces Apoptosis of Gastric Carcinoma SGC-7901 Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 13, Pages: 111-115(2017)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2017130111
  CLC： R285.5
- Published：2017
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GAO Qi-long, ZHU Ya-nan, SHI Bian, et al. Serum Containing Pericarp Inhibits Proliferation and Induces Apoptosis of Gastric Carcinoma SGC-7901 Cell[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(13): 111-115.
DOI：

GAO Qi-long, ZHU Ya-nan, SHI Bian, et al. Serum Containing Pericarp Inhibits Proliferation and Induces Apoptosis of Gastric Carcinoma SGC-7901 Cell[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(13): 111-115. DOI： 10.13422/j.cnki.syfjx.2017130111.

摘要

目的：探讨青龙衣含药血清对胃癌SGC-7901细胞增殖及凋亡的影响，并探讨其作用机制。方法：Wistar大鼠随机分为空白组、青龙衣低剂量（25 g·kg-1·d-1），中剂量（50 g·kg-1·d-1），高剂量（100 g·kg-1·d-1）组及顺铂（5 g·kg·d-1）组，连续灌胃给药7 d，空白组灌胃给予等体积生理盐水；末次给药2 h后，颈动脉采血，制备青龙衣含药血清；分别采用噻唑蓝（MTT）比色法、流式细胞术、烟酸己可碱（Hoechst）染色、实时荧光定量聚合酶链式反应（Real-time PCR）及蛋白免疫印迹法（Western blot）检测青龙衣含药血清对SGC-7901细胞增殖、凋亡率、凋亡指数、凋亡相关基因及凋亡信号通路蛋白表达的影响。结果：与空白组比较，青龙衣含药血清对SGC-7901细胞的增殖具有明显抑制作用（P<0.05），青龙衣含药血清处理48 h后的早期、晚期凋亡率，凋亡指数及促凋亡基因Bcl-2相关X蛋白（Bax），Bcl-2相关K蛋白（Bak）mRNA表达均升高，抗凋亡基因B淋巴细胞瘤-2（Bcl-2）mRNA表达降低（P<0.05）；青龙衣含药血清组β-链蛋白（β-catenin），细胞周期蛋白D1（Cyclin D1）及原癌基因（C-myc）蛋白表达均明显降低（P<0.05）。结论：青龙衣含药血清对胃癌SGC-7901细胞的增殖具有抑制作用，该作用可能与诱导凋亡及抑制分泌型糖蛋白（Wnt）/β-catenin通路的活化有关。

Abstract

Objective: To explore the effects of serum containing Juglans mandshurica (SCJM) pericarp on proliferation and apoptosis of gastric carcinoma SGC-7901 cells. Method: Wistar rats were randomly divided into blank control group

low (25 g·kg-1·d-1)

medium (50 g·kg-1·d-1) and high (100 g·kg-1·d-1) dose J.mandshurica treatment groups and cisplatin group (5 g·kg-1·d-1). The rats in treatment groups received lavage administration for 7 d

and the rats in blank control group received the equivalent volume of normal saline. 2 h after the last treatment

SCJM was prepared by carotid blood serum; cell proliferation

apoptosis rates

apoptosis index

levels of apoptosis related gene and protein expression were evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) colorimetry

flow cytometry

Hoechst staining

Real-time PCR and Western blot respectively. Result: SCJM could inhibit the proliferation of SGC-7901 cells (P<0.05) as compared with blank group

and 48 h after treatment by SCJM

early-and late-apoptosis rates

apoptosis index and mRNA levels of Bax and Bak were increased

but mRNA level of B-cell lymphoma-2 (Bcl-2) was decreased (P<0.05); the protein levels of β-catenin

Cyclin D1 and C-myc in SCJM-treatment groups were lower than those of control group (P<0.05). Conclusion: SCJM had inhibitory effect on proliferation of gastric carcinoma SGC-7901 cells

and the effect may be related to the induction of apoptosis and inhibition of Wnt/β-catenin pathway activation.

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