Separation and Identification of Flavonoids from

ZENG Ling-feng; XU Jun-wei; XU Li; ZHU Ling-li; LIU Hua

doi:10.13422/j.cnki.syfjx.2017140074

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Separation and Identification of Flavonoids from

更新时间：2024-01-04

- Separation and Identification of Flavonoids from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 14, Pages: 74-77(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017140074
  CLC： R284.1
- Published：2017
- 稿件说明：
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ZENG Ling-feng, XU Jun-wei, XU Li, et al. Separation and Identification of Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(14): 74-77.
DOI：

ZENG Ling-feng, XU Jun-wei, XU Li, et al. Separation and Identification of Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(14): 74-77. DOI： 10.13422/j.cnki.syfjx.2017140074.

摘要

目的：本课题组前期对自建的江西100种中草药提取物组分库样品进行活性筛选时，发现毛梗豨莶草Siegesbeckia glabresce的乙酸乙酯萃取部位具有显著的抑制细胞程序性坏死的生理活性。为明确毛梗豨莶草的活性组分，本课题组对毛梗豨莶草乙酸乙酯萃取部位进行系统化学成分分离，以其寻找具有抑制细胞程序性坏死的目标化合物。方法：采用正相硅胶柱色谱（二氯甲烷-甲醇，20∶1~0∶1），反相ODS柱色谱（30%~100%甲醇），Sephadex LH-20 柱色谱、重结晶等方法对毛梗豨莶草乙酸乙酯萃取部位进行分离和纯化，根据理化性质及1H-NMR，13 C-NMR波谱数据进行化合物结构鉴定。结果：此次报道从毛梗豨莶草中分离并纯化的9个黄酮类化合物，分别鉴定为：3，4-二甲氧基-2'，4'-二羟基查尔酮（1）；7-O-（β-D-glucopyranosyl）-galactin（2）；7，3'，4'-三羟基黄酮（3）；5，6，7，3'，4'，5'-六甲氧基黄酮（4）；8，3'，4'-三羟基-7-甲氧基二氢黄酮（5）；5，4'-二羟基-7，3'-二甲氧基二氢黄酮醇（6）；7，4'-二羟基-3'-甲氧基二氢黄酮（7）；木犀草素（8）；槲皮素（9）。结论：化合物1～7为首次从该植物中分离得到。

Abstract

Objective: To study the active chemical constituents of Siegesbeckia glabrescens

and find the target compounds with the function of inhibiting programmed cell necrosis. Method: The ethyl acetate extract fractions were isolated and purified by normal phase silica gel column chromatography (dichloromethane-methanol

20:1-0:1)

reverse phase ODS column chromatography (30%-100% methanol solution)

Sephadex LH-20 column chromatography and recrystallization; and their structures were determined according to physicochemical properties and spectral analysis. Result: Nine flavonoids were isolated and identified as 3

4-dimethoxy-2'

4'-dihydroxy chalcone (1)

7-O-(β-D-glucopyranosyl)-galactin (2)

4'-trihydroxy flavone (3)

5'-hexamethoxyflavone (4)

4'-trihydroxy-7-methoxy flavanoe (5)

4'-dihydroxy-7

3'-dimethoxy flavanoe (6)

4'-dihydroxy-3'-methoxyflavanone (7)

luteolin (8)

and quercetin (9). Conclusion: Compounds 1-7 were isolated from this plant for the first time.

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