Objective: Metabonomics was utilized to explore the effect of Epimedii Folium water extract(EWE) on the endogenous metabolism in the urine of normal rats

and to search for related metabolic pathways and potential targets to explore whether the differences of the biomarker's expression will affect the different functions of human systems along with the circular distribution of body fliuds

and then produce the potential intervention. Method: Rats have been randomly divided into the blank and EWE groups (10 rats per group) and been gavaged the same amount of physiological saline and EWE (87.3 mg·kg-1) for 20 days

respectively.The urine was gathered on the 21th days for analysis by UPLC-Q-TOF/MS. Result: Ten potential biomarkers and six major metabolic pathways were identified

and it found that EWE could protect the cardiovascular and cerebrovascular systems via elevating the expression of N

N'-bis(3-aminopropyl)-1

4-butanediamine and N-(3-aminopropyl)-1

4-butane-diamine.However

the EWE manifested adverse prevention from tumorous and neurological diseases through reduced expression of N-(ω)-hydroxy-L-arginine and β-tyrosine. Conclusion: EWE has a significantly protective effect on the cardiovascular and cerebrovascular diseases of normal rats;however

the potency and toxicity may coexist for the tumorous and neurological diseases.Its mechanisms are related to the metabolic pathway of amine oxide

tyrosine

sphingolipid and so on.