Objective: To discuss the influence of Guiqi Yiyuangao on the genetic expression of Bax

Bcl-2 and cytochrome-C(Cyt-C) in lung and kidney of rats damaged by heavy ion radiation induced bystander effect. Method: The Wistar male rats were randomly divided seven groups

the group of Chinese medicine in advance was given Guiqi Yiyuangao by gavage for two weeks. Later the right lung of the rats of the radiation group and the group of Chinese medicine was radiated by 12C6+ ion once. At 6

12

24 h after radiation all groups of rats were executed. The amounts of protein and mRNA expression of Bax

Bcl-2 and Cyt-C in right lung

left lung and left kidney were examined with Western blot

immunohistochemistry and Real-time PCR. Result: Compared with the normal control group

the amount of mRNA expression of Bax and Cyt-C in the right lung

left lung and left kidney of the radiation group obviously increases at 6

12

24 h after radiation. While the amount of protein expression of Bax and Cyt-C of the radiation group obviously increases at 24 h after radiation. There is statistically significant difference(P<0.01). The amount of protein and mRNA expression of Bcl-2 in the right lung of the radiation group obviously weakens at 24 h after radiation. But in the left lung and left kidney of the radiation group it obviously weakens at 6

12

and 24 h after radiation. There is statistically significant difference(P<0.01). Compared with the radiation group

the mRNA expression of Bax and Cyt-C of the Chinese medicine groups obviously down-regulates at 6

12

24 h after radiation. While the protein expression of the Chinese medicine group obviously down-regulates at 24 h after radiation. There is statistically significant difference(P<0.01). Compared with the radiation group

the expression of mRNA and protein of Bcl-2 in the right lung of the Chinese medicine group obviously up-regulates at 24 h after radiation. Compared with the radiation group

the expression of mRNA and protein of Bcl-2 in the left lung and left kidney of the Chinese medicine group obviously up-regulates at 6

12

24 h after radiation. There is statistically significant difference(P<0.01). Conclusion: It indicates that there are abnormal expressions of Bax

Bcl-2 and Cyt-C of lung and kidney in rats damaged by heavy ion radiation induced bystander effect. By delaying the down-regulation of the expression of Bcl-2

controlling the up-regulation of the expression of Bax and Cyt-C

Guiqi Yiyuangao can prevent the lung and kidney cell apoptosis and defend the damage caused by heavy ion radiation induced bystander effect in vivo.