Objective: To study on establishing the model of deficient heat syndrome due to insufficiency of Yin fluid in rats by Chinese medicinal herbs with heat property. Method: Different doses of Aconiti Lateralis Radix Praeparata

Zingiberis Rhizoma and Cinnamomi Cortex were administered intragastrically to rats.Rats' ordinary situation was observed

included active state

hair luster

claw color

urine and stool.Rectal temperature

food intake

water intake

urine output and fecal amount were recorded at the same time in every day

the changes of triiodothyronine(T3) and thyroxine(T4) in serum

cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate(cGMP) in plasma and indexes of relevant organs were detected when body fluid consumption appeared obvious difference. Result: The water intake and body fluid consumption of model groups were mostly increased by comparing with those of the control group

rats showed manifestations of heat syndrome due to insufficiency of Yin fluid.T4 in serum showed a increasing trend

but T3/T4 levels showed the trend of decline.Compared with the control group

most of cAMP and cGMP in the low and middle dose of model groups increased significantly

while the high dose of model group decreased. Conclusion: Deficient heat syndrome model can be established by administering Chinese medicinal herbs with heat property

increasing of body fluid consumption has some guiding significance for replication of deficient heat syndrome model.