Effect of Danshen Injection on PI3K/Akt/mTOR Pathway and Lower Extremity Motor Function in Rats With Spinal Cord Injury

LIU Yang; GAO Yu-ting; MIAO Yu-chuan

doi:10.13422/j.cnki.syfjx.2017140152

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Effect of Danshen Injection on PI3K/Akt/mTOR Pathway and Lower Extremity Motor Function in Rats With Spinal Cord Injury

更新时间：2024-01-04

- Effect of Danshen Injection on PI3K/Akt/mTOR Pathway and Lower Extremity Motor Function in Rats With Spinal Cord Injury
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 14, Pages: 152-157(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017140152
  CLC： R285.5
- Published：2017
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LIU Yang, GAO Yu-ting, MIAO Yu-chuan. Effect of Danshen Injection on PI3K/Akt/mTOR Pathway and Lower Extremity Motor Function in Rats With Spinal Cord Injury[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(14): 152-157.
DOI：

LIU Yang, GAO Yu-ting, MIAO Yu-chuan. Effect of Danshen Injection on PI3K/Akt/mTOR Pathway and Lower Extremity Motor Function in Rats With Spinal Cord Injury[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(14): 152-157. DOI： 10.13422/j.cnki.syfjx.2017140152.

摘要

目的：探讨脊髓损伤（SCI）后丹参注射液促进大鼠脊髓神经功能恢复的机制。方法：大鼠（清洁级）随机分为正常组、模型组、给药组、信号通路阻断组和空白组（n=10）。除正常组外，其余各组大鼠均建立重力打击SCI模型。模型组伤后常规饲养；给药组伤后1 h腹腔注射丹参注射液（1 mL·kg-1）；信号通路阻断组伤后1 h腹腔注射丹参注射液（1 mL·kg-1，含雷帕霉素 3 mg·kg-1）；空白组伤后给予腹腔注射生理盐水（1 mL·kg-1）。上述各组均按1次/d注射。14 d后处死动物，处死前采用联合行为评分法（CBS）评价大鼠脊髓神经功能恢复情况，并取损伤节段上下1 cm的脊髓组织。分别采用免疫组化SP法，蛋白质免疫印迹（Western blot）和逆转录-聚合酶链式反应（RT-PCR）技术检测各实验组大鼠PI3K，Akt和mTOR蛋白及mRNA的表达水平。结果：与模型组、信号通路阻断组和空白组大鼠比较，给药组大鼠CBS评分显著降低（P<0.05）；与正常组比较，模型组大鼠PI3K，Akt，mTOR mRNA的含量，以及PI3K，Akt，mTOR蛋白免疫阳性细胞数和表达量均升高（P<0.05）；与模型组比较，给药组大鼠的上述指标均显著升高（P<0.05）；与给药组比较，信号通路阻断组大鼠的上述指标均显著降低（P<0.05）；上述指标在模型组与空白组大鼠之间的差异不具有统计学意义。结论：丹参注射液有助于大鼠SCI后脊髓神经功能的恢复，其机制可能与升高PI3K/Akt/mTOR信号转导通路的活性有关。

Abstract

Objective: To observe the effect of Danshen injection on promoting the recovery of spinal nerve function in rats after spinal cord injury (SCI) and investigate its action mechanism. Method: Fifty SPF SD rats were divided into five groups randomly: normal control group

injury group

treatment group

inhibition group and blank control group (n=10 in each group). All the rats except those in inormal control group received modified Allen's method to establish SCI models. The rats in njury group were fed routinely after injury; Danshen injection (1 mL·kg-1) was intraperitoneally injected 1 h after injury in treatment group; Danshen injection(1 mL·kg-1

including rapamycin 3 mg·kg-1) was intraperitoneally injected 1 h after injury in inhibition group; and normal saline (1 mL·kg-1) was intraperitoneally injected into the rats in blank control group after injury

once a day respectively. The rats in all groups were killed on 14 d

and the combine behavioral scores (CBS) of rats in all groups were detected before being killed to evaluate the recovery of spinal nerve function; and then the spinal cord tissues 1 cm above and below the injured segments were taken. Immunohistochemical SP method

Western blot and reverse transcription polymerase chain reaction (RT-PCR) were used respectively to detect PI3K

Akt and mTOR protein and mRNA expression levels. Result: After treatment

the motor function of posterior limbs (CBS score) in treatment group was better than that in injury group

inhibition group and blank control group (P<0.05); PI3K

Akt and mTOR mRNA expression levels

immunoreactive cells count and relative expression levels in treatment group were also significantly higher than those in other groups (P<0.05). Conclusion: Danshen injection could promote the recovery of nerve function after SCI probably by elevating the activity of PI3K/Akt/mTOR signaling-pathway.

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