Analysis on Variation of Fatty Oil Components in Vaccariae Semen,Cassiae Semen and Ziziphi Spinosae Semen Before and After Stir-frying by GC-MS

CAI Jin-jin; CHEN Lu

doi:10.13422/j.cnki.syfjx.2017150031

您当前的位置：

首页 >

文章列表页 >

Analysis on Variation of Fatty Oil Components in Vaccariae Semen,Cassiae Semen and Ziziphi Spinosae Semen Before and After Stir-frying by GC-MS

更新时间：2024-01-04

- Analysis on Variation of Fatty Oil Components in Vaccariae Semen,Cassiae Semen and Ziziphi Spinosae Semen Before and After Stir-frying by GC-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 15, Pages: 31-34(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017150031
  CLC： R283.3
- Published：2017
- 稿件说明：
移动端阅览
CAI Jin-jin, CHEN Lu. Analysis on Variation of Fatty Oil Components in Vaccariae Semen,Cassiae Semen and Ziziphi Spinosae Semen Before and After Stir-frying by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(15): 31-34.
DOI：

CAI Jin-jin, CHEN Lu. Analysis on Variation of Fatty Oil Components in Vaccariae Semen,Cassiae Semen and Ziziphi Spinosae Semen Before and After Stir-frying by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(15): 31-34. DOI： 10.13422/j.cnki.syfjx.2017150031.

摘要

目的：分析和鉴定王不留行、决明子、酸枣仁3种药材炒制前后脂肪油类成分的变化，为探索种子类药材“逢子必炒”的炮制机制奠定基础。方法：以石油醚为溶剂，采取索氏提取器提取脂肪油，甲酯化后用气相色谱-质谱联用技术（GC-MS）分离、鉴定其组成和相对质量分数。HP-5毛细管柱（0.25 mm×30 m，0.25 μm），载气99.999%高纯氮，流速1 mL·min-1，采用程序升温方式（初始温度50 ℃，以5 ℃·min-1升至170 ℃，停留4 min，5 ℃·min-1升至190 ℃，0.5 ℃·min-1升至200 ℃，10 ℃·min-1升至240 ℃，保持4 min），进样量1 μL，电子轰击离子源（EI），离子源温度220 ℃，电子能量70 eV。结果：王不留行、决明子、酸枣仁3种药材生品脂肪油提取率分别为3.34%，6.16%，20.66%，炒制品脂肪油提取率分别为4.67%，7.40%，26.35%。王不留行生品鉴定出7个化合物，炒制品鉴定出10个化合物；决明子生品鉴定出8个化合物，炒制品鉴定出9个化合物；酸枣仁生品鉴定出10个化合物，炒制品鉴定出14个化合物。结论：炒制能提高脂肪油的提取效率，炒制后3种药材脂肪油的组成出现了一些共性变化。

Abstract

Objective: To explore the mechanism of the theory of “every semen must be fried” by analyzing and identifying the variation of fatty oil components in Vaccariae Semen

Cassiae Semen and Ziziphi Spinosae Semen before and after stir-frying. Method: The fatty oil was extracted through petroleum ether by Soxhlet extractor

the composition and relative content was analyzed and identified by GC-MS after methyl esterification

the analysis was performed on a HP-5 capillary column

the carrier gas was nitrogen

the column temperature was set at 50 ℃ and elevated to 170 ℃ at 5 ℃·min-1

keeping 4 min

then up to 190 ℃ at the rate of 5 ℃·min-1

up to 200 ℃ at the rate of 0.5 ℃·min-1

up to 240 ℃ at the rate of 10 ℃·min-1

keeping 4 min.Inject volume was 1 μL.The ionization mode was electron ionization

the ion source temperature was 220 ℃

the electron energy was 70 eV. Result: The extraction rates of fatty oil in Vaccariae Semen

Cassiae Semen and Ziziphi Spinosae Semen were 3.34%

6.16% and 20.66%

the extraction rates of fatty oil in their stir-frying products were 4.67%

7.40% and 26.35%

respectively.Seven compounds in crude products of Vaccariae Semen were identified

and ten compounds in its stir-frying products of Vaccaroae Semen were identified;eight compounds in crude products of Cassiae Semen were identified

nine compounds in its stir-frying products of Cassiae Semen were identified;ten compounds in crude products of Zizyphi Spinosae Semen were identified

fourteen compounds in its stir-frying products of Zizyphi Spinosae Semen were identified. Conclusion: The extraction rate of fatty oil in these three Chinese herbal medicines are increased after stir-frying

the composition of fatty oil in them has some common change after stir-frying.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Impact of Maxing Kugan Decoction on Inflammatory Response and Apoptosis in Oleic Acid-induced Acute Lung Injury in Rats via p38 MAPK/NF-κB Signaling Pathway

Implications of Approval of Cassiae Semen-based Health Food Products for Development and Supervision of Substances with both Edible and Medicinal Values

Simultaneous Identification of Ziziphi Spinosae Semen and Its Adulterants by Multiplex Allele-specific PCR

Analysis of Rancidity Mechanism of Armeniacae Semen Amarum During Storage

Research Progress of Comprehensive Utilization of Ziziphi Spinosae Semen and Its By-products

Related Author

JIAO Taiqiang

NAN Yi

YUAN Ling

LI Jiaqing

NIU Yang

DU Peng

WANG Dan

TIAN Jixiang

Related Institution

College of Pharmacy，Ningxia Medical University

Ningxia Regional Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Regional High Incidence Disease，Ningxia Medical University

Ningxia Regional Key Laboratory of Ministry of Education of Traditional Chinese Medicine for Prevention and Treatment of Regional High Incidence Disease，Ningxia Medical University

School of Traditional Chinese Medicine，Ningxia Medical University

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰