Objective: To explore the optimal extraction procedures of soluble components (water-soluble and liposoluble components) of Cervi Cornu Pantotrichum

and analyze their content variations by using Chinese deer antlers. Method: The optimal extraction method of the water-soluble components was obtained by comparing three different fragmentation methods (ultrasonic extraction method

bullet blender cell and tissue fragmentation method and Jingxin tissue and cell grind extraction method) coupled with gel filtration chromatography (GFC). The more suitable measurement method for liposoluble components was determined by comparing two pre-treatment methods (105 ℃ drying method and 24 h freeze-drying method) coupled with soxhlet extraction. Finally

an optimal extraction project was adopted to measure the soluble components between the tip and basal portions of sika deer two-branch antlers

sika deer three-branch antlers and wapiti antlers. Result: The highest extraction efficiency of water-soluble components in Cervi Cornu Pantotrichum was obtained when Bullet Blender cell and tissue fragmentation method was used and the parameter was set at third grade

3 mins for 3 times. The extraction of liposoluble components was stable when 105 ℃ drying method was used

and the relative deviation was less than 5%. The contents of soluble components in the tip and basal portions of different kinds of antlers were 36.06% and 8.30% for sika deer two-branch antlers; 34.54% and 7.20% for sika deer three-branch antlers; 32.42% and 3.91% for wapiti antlers. Conclusion: We successfully determined the best extraction methods for water-soluble and liposoluble components in velvet antlers

and found that the soluble components of different kinds of deer antlers in the tip portions were significantly higher than those in the basal portions. The results showed a descending trend in sika deer two-branch antlers

sika deer three-branch antlers and wapiti antlers as well

but the differences were not significant.