Objective: To study on the expression of genes involved in the biosynthesis of terpenoid secondary metabolism

and explore the functional genes in Aconitum carmichaelii based on transcriptomics sequencing analysis of six A. carmichaelii organs (including main root

lateral root

stem

leaves

flower and fruit). Method: Illumina Hiseq 2500 was used to detect the sequencing and assemble into unigennes. By comparing with public database

the genes were annotated and differential expressed genes were obtained. Subsequently

Real-time PCR method was used to verify the key genes involved in the terpenoid secondary metabolism biosynthesis. Result: Total 156 967 635 Clean Reads (28 254 174 300 bp) were obtained

and 103 337 unigenes were obtained after sequence merging. Homology analysis of nucleotide and protein sequences indicated that 37.31% (38 554 unigenes) had different degree of homology with other creatures. In analysis of the functional classification and metabolic pathway (KEGG)

158 unigenes (encoding 5 enzymes) were involved in terpenoid synthesis. Conclusion: These genes will provide the basic data for the terpenoids biosynthetic pathway and molecular mechanism of A. carmichaelii.