GAO Bo, WEI Xiao-lu, HAN Ling-yu, et al. Research on Isolation of Bufadienolides from Cinobufacin Injection and Its Activity Screening of Anti-cancer and [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 78-84.
DOI:
GAO Bo, WEI Xiao-lu, HAN Ling-yu, et al. Research on Isolation of Bufadienolides from Cinobufacin Injection and Its Activity Screening of Anti-cancer and [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 78-84. DOI: 10.13422/j.cnki.syfjx.2017160078.
Research on Isolation of Bufadienolides from Cinobufacin Injection and Its Activity Screening of Anti-cancer and
Objective: According to observation of the anti-cancer effect of bufadienolides from cinobufacin injection in vivo and in vitro and the anti-pancreatic cancer cells of bufadienolide monomers in vitro
to indentify the foundation of bufadienolides on anti-pancreatic cancer. Method: Modern chromatographic separation technology was adopted to obtain bufadienolides from cinobufacin injection
HPLC-DAD-FT-ICR-MS was employed to analysis and identify the main monomers of bufadienolides.Anti-cancer activity of bufadienolides was screened by in vitro cells
then setting up the pancreatic cancer animal model in nude mice in vivo for evaluating the efficacy of bufadienolides
the anti-pancreatic cancer activity of the main bufadienolide monomers in vitro was screened. Result: IC50 of bufadienolides from cinobufacin injection on hepatocellular carcinoma cells(BEL7402)
gastric carcinoma cells(BGC823) and pancreatic cancer cells (SW1990 and MIA PaCa-2) in vitro were (0.47±0.03)
(0.06±0.01)
(0.06±0.02)
(0.11±0.03) g· L-1
respectively.LD50 of bufadienolides was 20 mg·kg-1 and the treatment effect on SW1990 nude mice in vivo was close to gemcitabine.Gamabufotalin
arenobufagin and hellebrigenin had obvious inhibitory effect on SW1990 and BxPC-3 cells
which were isolated and identified from bufadienolides. Conclusion: Bufadienolides from cinobufacin injection shows almost the same inhibition rate on pancreatic cancer cells as gastric cancer in vitro
and it has apparently treatment effect on human pancreatic cancer bearing xenograft nude mice in vivo.Gamabufotalin
arenobufagin and hellebrigenin from bufadienolides show significantly inhibition on pancreatic cancer cells in vitro.