CUI Ya-hui, YAO Jun-na, ZHANG Zhi-jie, et al. Quality Evaluation of Decoction Pieces and Water Extracts of Genkwa Ramulus by Infrared Spectroscopy and Chromatography Technology[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 85-89.
DOI:
CUI Ya-hui, YAO Jun-na, ZHANG Zhi-jie, et al. Quality Evaluation of Decoction Pieces and Water Extracts of Genkwa Ramulus by Infrared Spectroscopy and Chromatography Technology[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 85-89. DOI: 10.13422/j.cnki.syfjx.2017160085.
Quality Evaluation of Decoction Pieces and Water Extracts of Genkwa Ramulus by Infrared Spectroscopy and Chromatography Technology
Objective: To study the decoction pieces and water extracts of Genkwa Ramulus by using high performance liquid chromatography (HPLC) and Tri-Step infrared identification method. Method: The 10 batches of crude drugs of Genkwa Ramulus were collected; their decoction pieces and water extracts were prepared separately. Tri-Step infrared spectroscopy was used to analyze various batches of decoction pieces and water extracts; HPLC was used to determine the content of daphnetin. The data were analyzed to determine the characteristic spectrum of Genkwa Ramulus decoction pieces and water extracts
and then the component differences and similarities between these two forms were investigated. Result: From the one-dimensional spectroscopy
Genkwa Ramulus decoction pieces and water extracts showed maximum absorption at 1 055 and 1 073 cm-1 respectively. From the second derivative spectroscopy
the results of Genkwa Ramulus water extracts (1 707
1 650 and 1 624 cm-1) were asically consistent with those of daphnetin (1 709
1651 and 1 625 cm-1). Two-dimensional correlation infrared spectroscopy showed that both Genkwa Ramulus decoction pieces and water extracts had three obvious auto-peaks at different regions. From HPLC
the results showed that the content of daphnetin in the water extracts was significantly higher than that in decoction pieces
indicating that this kind of components can be effectively enriched after water extraction. Conclusion: Tri-Step infrared spectroscopy provided the overall composition information of Genkwa Ramulus decoction pieces and water extracts; two dimensional infrared spectroscopy can be used for the identification of these two forms; and HPLC was used to detect the content of main effective compositions and further explain the changes of compositions.
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JIA Zhe
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ZHANG Cun
ZHU Ying
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Related Institution
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
School of Pharmacy, Henan University of Chinese Medicine
State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine
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