Effect of Combined Prescription of Linggui Zhugan Tang and Yinchenhao Tang on Nrf2/ARE Signaling Pathway in Rats with Non-alcoholic Steatohepatitis

GUO Yi; LI Jun-xiang; MAO Tang-you; CHEN Chen; XIE Tian-hong; HAN Ya-fei; SHI Lei; TAN Xiang; HAN Hai-xiao

doi:10.13422/j.cnki.syfjx.2017160108

您当前的位置：

首页 >

文章列表页 >

Effect of Combined Prescription of Linggui Zhugan Tang and Yinchenhao Tang on Nrf2/ARE Signaling Pathway in Rats with Non-alcoholic Steatohepatitis

更新时间：2024-01-04

- Effect of Combined Prescription of Linggui Zhugan Tang and Yinchenhao Tang on Nrf2/ARE Signaling Pathway in Rats with Non-alcoholic Steatohepatitis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 16, Pages: 108-113(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017160108
  CLC： R285.5
- Published：2017
- 稿件说明：
移动端阅览
GUO Yi, LI Jun-xiang, MAO Tang-you, et al. Effect of Combined Prescription of Linggui Zhugan Tang and Yinchenhao Tang on Nrf2/ARE Signaling Pathway in Rats with Non-alcoholic Steatohepatitis[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 108-113.
DOI：

GUO Yi, LI Jun-xiang, MAO Tang-you, et al. Effect of Combined Prescription of Linggui Zhugan Tang and Yinchenhao Tang on Nrf2/ARE Signaling Pathway in Rats with Non-alcoholic Steatohepatitis[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 108-113. DOI： 10.13422/j.cnki.syfjx.2017160108.

摘要

目的：从氧化应激的角度，观察苓桂术甘汤与茵陈蒿汤合方对非酒精性脂肪性肝炎（NASH）大鼠的干预效应，并通过Nrf2/ARE信号通路探讨合方对非酒精性脂肪性肝炎（NASH）大鼠的作用机制。方法：将SPF级SD大鼠随机分为正常组，模型组，合方组（6.93 g·kg-1），苓桂术甘汤组（3.465 g·kg-1），茵陈蒿汤组（3.465 g·kg-1），莱菔硫烷组（0.5 mg·kg-1），采用高脂饲料法建立NASH模型；高脂饲料饲养8周并同时给药，取材检测大鼠血清丙氨酸氨基转移酶（ALT），天门冬氨酸氨基转移酶（AST）以及血脂总胆固醇（total cholesterol，TC），甘油三酯（triglyceride，TG），高密度脂蛋白胆固醇（high-density lipoprotein，HDL-C），低密度脂蛋白胆固醇（low density lipoprotein，LDL-C）水平，取部分肝组织进行苏木素-伊红（HE）染色，蛋白质免疫印迹（Western blot）与逆转录PCR（RT-PCR）法测定肝脏细胞质接头蛋白1（Kelch-like ECH-associated protein 1，Keap1），核因子E2-关联因子2（nuclear factor E2-related factor 2，Nrf2），醌氧化还原酶1（quinone oxidoreductase-1，NQO1），血红素加氧酶1（heme oxygenase-1，HO-1）蛋白与mRNA表达水平。结果：给药后，与正常组比较，模型组大鼠血脂TC，TG，HDL-C，LDL-C及血清ALT，AST水平升高，肝脏脂肪变明显，肝脏Keap1表达量降低，Nrf2，NQO1，HO-1蛋白与mRNA表达量升高（P <0.05）；与模型组比较，各给药组血脂TC，TG，HDL-C，LDL-C及血清ALT，AST水平有不同程度降低，肝脏病理学也有不同程度改善，除茵陈蒿汤组变化不明显外，各给药组大鼠肝脏Nrf2，NQO1，HO-1蛋白与mRNA表达量均有不同程度升高（P <0.05，P <0.01）；但对于Keap1的表达无明显作用。结论：苓桂术甘汤以及合方可能通过激活Nrf2/ARE信号通路，从而达到改善氧化应激，防治非酒精性脂肪性肝炎的目的。

Abstract

Objective: To observe the effects of combined prescription of Linggui Zhugan Tang (LGZGT) and Yinchenhao Tang (YCHT) on Nrf2/ARE signaling pathway in rats with non-alcoholic steatohepatitis (NASH) from the perspective of oxidative stress

and investigate its mechanism for treating non-alcoholic steatohepatitis. Method: SPF grade SD rats were randomly divided into normal control group

model group

sulforaphane group (0.5 mg·kg-1)

LGZGT group (3.465 g·kg-1)

YCHT group (3.465 g·kg-1) and LGZGT+YCHT combination group (6.93 g·kg-1). High-fat diet was given to establish NASH models

and the treatment was given at the same time for 8 weeks. Then the serum was harvested separately to detect the levels of alanine aminotransferase (ALT)

aspartate aminotransferase (AST)

total cholesterol (TC)

triglyceride (TG)

high-density lipoprotein (HDL-C)

and low density lipoprotein (LDL-C); part liver tissues were taken for HE staining; Western blot and reverse transcription PCR were used respectively to detect the protein and mRNA expression levels of Kelch-like ECH-associated protein 1 (Keap1)

nuclear factor E2-related factor 2 (Nrf2)

quinone oxidoreductase-1 (NQO1)

and heme oxygenase-1 (HO-1). Result: As compared with normal control group

the levels of TC

HDL-C and LDL-C in blood lipid and ALT

AST levels in serum were increased;liver fat became obvious; the expression level of Keap1 in liver was decreased; and

Nrf2

NQO1

HO-1 protein and mRNA expression levels were increased in the model group (P <0.05).As compared with the model group

the levels of TC

HDL-C and LDL-C in blood lipid and ALT

AST levels in serum were decreased in various treatment groups

liver pathology was also improved to some extent; and Nrf2

NQO1

HO-1 protein and mRNA expression levels were increased in treatment groups (P <0.01

P <0.05) except YCHT group. However

the levels of Keap1 didn't show obvious differences among the treatment groups. Conclusion: LGZGT and its combination formula may improve oxidative stress to prevent and treat NASH via activating Nrf2/ARE signaling pathway in the liver.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of Non-alcoholic Steatohepatitis Improved by Qizhu Prescription Based on AMPK Signaling Pathway

Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma Ameliorates Myocardial Ischemia-reperfusion Injury in Rats via FUNDC1-dependent Mitophagy

Shengxiantang Alleviates Idiopathic Pulmonary Fibrosis in Rats by Regulating Oxidative Stress to Inhibit PANoptosis

Shugan Bushen Yulin Decoction Improves Testicular Spermatogenic Function in Rat Model of Oligoasthenospermia via IRS/Akt/FoxO1 Signaling Pathway

Related Author

ZHANG Qiuyi

ZHOU Zhenhua

ZHANG Qiuyi

ZHOU Zhenhua

WANG Zhe

WANG Guoliang

WANG Ziyi

HAO Qianying

Related Institution

Shuguang Hospital，Shanghai University of Traditional Chinese Medicine（TCM）

Anhui Hospital， Shuguang Hospital， Shanghai University of TCM

Shuguang Hospital， Shanghai University of Traditional Chinese Medicine

Anhui Hospital， Shuguang Hospital， Shanghai University of Traditional Chinese Medicine

Guang'anmen Hospital， China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰