miRNA Expression Analysis in AMI Rat BMSCs Intervened by Active Principle Region of Yangxin Tongmai Formula

ZHU Zi-ming; ZHENG Jing-hui; HUANG Jin-long; CHEN Jian-jun; MO Yun-qiu; HUANG Xiu-jie; MA Xiao-cong; ZHANG Jian; LUO Wei; WU Yan-hong

doi:10.13422/j.cnki.syfjx.2017160114

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miRNA Expression Analysis in AMI Rat BMSCs Intervened by Active Principle Region of Yangxin Tongmai Formula

更新时间：2024-01-04

- miRNA Expression Analysis in AMI Rat BMSCs Intervened by Active Principle Region of Yangxin Tongmai Formula
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 16, Pages: 114-120(2017)
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- DOI：10.13422/j.cnki.syfjx.2017160114
  CLC： R285.5
- Published：2017
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ZHU Zi-ming, ZHENG Jing-hui, HUANG Jin-long, et al. miRNA Expression Analysis in AMI Rat BMSCs Intervened by Active Principle Region of Yangxin Tongmai Formula[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 114-120.
DOI：

ZHU Zi-ming, ZHENG Jing-hui, HUANG Jin-long, et al. miRNA Expression Analysis in AMI Rat BMSCs Intervened by Active Principle Region of Yangxin Tongmai Formula[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 114-120. DOI： 10.13422/j.cnki.syfjx.2017160114.

摘要

目的：通过筛选差异表达miRNAs，预测和探究养心通脉方有效部位方（active principle region of Yangxing Tongmai formula，apr-YTF）诱导骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）心肌样分化的分子机制。方法：提取急性心肌梗死（acute myocardial infarction，AMI）血瘀证大鼠BMSCs并培养，使用apr-YTF含药血清诱导，以阴性对照血清做对照，提取细胞总RNAs，使用表达谱芯片检测细胞miRNAs的表达，并进行聚类分析。运用生物信息学方法预测差异miRNA调控的靶基因和功能分析。结果：芯片探测出共有26个差异miRNAs，其中8个上调，18个下调，经筛选和预测共100个靶基因参与了19种生物过程，11种细胞成分，10种分子作用。轴突导向通路，癌症转录误调节通路，AMPK信号通路被富集。rno-miR-93-5p，rno-miR-204-5p，rno-miR-128-3p为网络调控中心，其中rno-miR-204-5p下调更显著。结论：养心通脉有效部位方干预大鼠骨髓间充质干细胞心肌样分化的机制可能与rno-miR-204-5p，rno-miR-93-5p，轴突导向通路相关性较高。

Abstract

Objective: To predict and investigate the molecular mechanism of active principle region of Yangxing Tongmai formula (apr-YTF) in inducing bone marrow mesenchymal stem cells (BMSCs) myocardial differentiation based on miRNA level. Method: BMSCs from acute myocardial infarction (AMI) rats of blood-stasis model were extracted and cultivated. Then apr-YTF serum was used as experimental group and negative control serum was used as control group

respectively. Total RNAs were extracted and differently expressed miRNAs were detected by expression profile chip for hierarchical clustering analysis. Bioinformatics method was used to predict target genes and analyze the functions of differential miRNAs regulation. Result: There were a total of 26 differently expressed miRNAs

8 were up-regulated

and 18 were down-regulated. In screening and predicting

100 target genes were involved in 19 kinds of biological processes

11 kinds of cellular components

and 10 kinds of molecular functions. Axon guidance

Transcriptional misregulation in cancer

and AMPK signaling pathway were enriched. Rno-miR-93-5p

rno-miR-204-5p

rno-miR-128-3p were the center of the molecular network

and rno-miR-204-5p was down-regulated significantly. Conclusion: The results showed that rno-miR-204-5p

rno-miR-93-5p and Axon guidance pathway may play a role in the differentiation process of BMSCs into cardiomyocytes induced by apr-YTF.

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