Anti-liver Fibrosis Effect of Ganlong Capsule Combined with Compound Glycyrrhizin Glucoside

SHAO Ming-yuan; JIAO Su-yue; YANG Qiang-li; ZHOU Xuan; CAO Chang-e; LAI Yong

doi:10.13422/j.cnki.syfjx.2017160133

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Anti-liver Fibrosis Effect of Ganlong Capsule Combined with Compound Glycyrrhizin Glucoside

更新时间：2024-01-04

- Anti-liver Fibrosis Effect of Ganlong Capsule Combined with Compound Glycyrrhizin Glucoside
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 16, Pages: 133-138(2017)
- 作者机构：
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- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017160133
  CLC： R285
- Published：2017
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SHAO Ming-yuan, JIAO Su-yue, YANG Qiang-li, et al. Anti-liver Fibrosis Effect of Ganlong Capsule Combined with Compound Glycyrrhizin Glucoside[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 133-138.
DOI：

SHAO Ming-yuan, JIAO Su-yue, YANG Qiang-li, et al. Anti-liver Fibrosis Effect of Ganlong Capsule Combined with Compound Glycyrrhizin Glucoside[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 133-138. DOI： 10.13422/j.cnki.syfjx.2017160133.

摘要

目的：通过检测肝龙胶囊联合复方甘草酸苷用药后人肝星状细胞系（LX-2）中I型胶原（Col-I），III型前胶原（PCIII），IV型胶原（IV-C），层粘蛋白（LN），透明质酸（HA），转化生长因子-β1（TGF-β1）的含量及Col-I，基质金属蛋白酶抑制剂1（TIMP-1） mRNA的表达变化，探讨肝龙胶囊联合复方甘草酸苷对LX-2细胞的抗肝纤维化作用。方法：体外培养LX-2细胞，将对数期细胞分为复方甘草酸苷用药组（3.125，1.562，0.781 g·L-1），空白组，肝龙胶囊（0.938，0.469，0.235 g·L-1）联合复方甘草酸苷（3.125，1.562，0.781 g·L-1）交叉用药组，采用酶联免疫吸附测定（ELISA）法检测肝纤维化四项指标PCIII，IV-C，LN，HA和Col-I，TGF-β1的含量；逆转录PCR（RT-PCR）检测Col-I和TIMP-1 mRNA的表达水平。结果：与复方甘草酸苷用药组比较，联合用药组在24，48 h时均可使HA，Col-I的含量降低（P <0.05），在72 h可使LN，HA，Col-I，PCIII，TGF-β1的含量降低（P <0.05）；与空白组比较，联合用药组可使TGF-β1含量明显降低（P <0.05，P <0.01）；与复方甘草酸苷用药组比较，联合用药组明显降低TIMP-1 mRNA的表达量（P <0.05），且呈现时间依赖性；与空白组比较，联合用药组对Col-I mRNA在24 h呈现抑制作用（P <0.05），在72 h时却使其表达量增加。结论：肝龙胶囊联合复方甘草酸苷用药组可使LX-2细胞LN，HA，Col-I，TGF-β1的含量降低，抑制Col-I和TIMP-1 mRNA的表达，效果优于复方甘草酸苷用药组。肝龙胶囊增强了复方甘草酸苷的体外抗肝纤维化作用。

Abstract

Objective: To observe the anti-liver fibrosis effect of Ganlong capsule combined with compound glycyrrhizin glucoside on human hepatic stellate cells by detecting the content of type I collagen (Col-I)

type III procollagen (PCIII)

type IV collagen (IV-C)

laminin (LN)

hyaluronic acid (HA)

transforming growth factor-β1 (TGF-β1) and expression levels of Col-I

matrix metalloproteinase inhibitor 1 (TIMP-1) mRNA in human hepatic stellate cell line (LX-2). Method: LX-2 cells were cultured in vitro

and the logarithmic phase cells were divided into compound glycyrrhizin glucoside treatment groups (3.125

1.562

0.781 g·L-1)

blank control group

Ganlong capsule (0.938

0.469

0.235 g·L-1) combined with compound glycyrrhizin glucoside (3.125

1.562

0.781 g·L-1) groups. Then enzyme-linked immunosorbent assay (ELISA) method was used to detect the content of four markers of liver fibrosis (PCIII

IV-C

HA) and the levels of Col-I and TGF-β1; the expression levels of Col-I and TIMP-1 mRNA were detected by reverse transcription-PCR (RT-PCR). Result: As compared with compound glycyrrhizin glucoside treatment group

combined drug use could decrease the content of Col-I

HA at 24 h and 48 h (P <0.05)

and reduce the content of LN

Col-I

PCIII

TGF-β1 at 72 h (P <0.05). As compared with the blank control group

the combined drug use can significantly decrease the content of TGF-β1 (P <0.05

P <0.01). As compared with compound glycyrrhizin glucoside treatment group

the combined drug use could significantly decrease the expression of TIMP-1 mRNA (P <0.05) in time-dependent manner; and as compared with the blank control group

the combined drug use showed the inhibition effect for Col-I mRNA at 24 h (P <0.05)

but the expression was increased at 72 h. Conclusion: Ganlong capsule combined with compound glycyrrhizin glucoside can decrease the content of LN

Col-I

TGF-β1 and inhibit the expression of Col-I and TIMP-1 in LX-2 cells

and the effect was better than that of compound glycyrrhizin glucoside treatment group. Ganlong capsule could enhance the anti-fibrosis effect in vitro of compound glycyrrhizin glucoside.

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