Effect of Qutan Huoxue Granules on Expression of AQP7 and p38MAPK in Adipose Tissues of NAFLD Model Rats

YANG Xiang-xiang; ZHU Xiao-ning; PENG Meng-yun; WANG Jing

doi:10.13422/j.cnki.syfjx.2017160145

您当前的位置：

首页 >

文章列表页 >

Effect of Qutan Huoxue Granules on Expression of AQP7 and p38MAPK in Adipose Tissues of NAFLD Model Rats

更新时间：2024-01-04

- Effect of Qutan Huoxue Granules on Expression of AQP7 and p38MAPK in Adipose Tissues of NAFLD Model Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 16, Pages: 145-151(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017160145
  CLC： R-332;R285.5
- Published：2017
- 稿件说明：
移动端阅览
YANG Xiang-xiang, ZHU Xiao-ning, PENG Meng-yun, et al. Effect of Qutan Huoxue Granules on Expression of AQP7 and p38MAPK in Adipose Tissues of NAFLD Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 145-151.
DOI：

YANG Xiang-xiang, ZHU Xiao-ning, PENG Meng-yun, et al. Effect of Qutan Huoxue Granules on Expression of AQP7 and p38MAPK in Adipose Tissues of NAFLD Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 145-151. DOI： 10.13422/j.cnki.syfjx.2017160145.

摘要

目的：探讨祛痰活血颗粒治疗非酒精性脂肪性肝病（NAFLD）大鼠的作用，并探讨其相关的作用机制。方法： 40只SD大鼠随机分成正常组（6只），模型组（34只）；除正常组外，采用高脂饮食诱导的方法诱导NAFLD大鼠模型，确认造模成功后，将剩余模型组（30只）随机分为5组，每组6只，分别为模型组，祛痰活血颗粒高、中、低剂量组（10，5，2.5 g·kg-1），SB203580组（3 mg·kg-1），分别予以灌胃祛痰活血颗粒及腹腔注射p38MAPK抑制剂SB203580，连续干预4周。苏木素-伊红（HE）及油红O染色观察肝脏病理变化；试剂盒测定血清甘油三酯（TG），总胆固醇（TC），天门冬氨酸氨基转移酶（AST），丙氨酸氨基转移酶（ALT），肝脏TG；实时荧光定量PCR（Real-time PCR）检测脂肪组织水通道蛋白7（AQP7）及p38分裂原激活的蛋白激酶（p38MAPK）mRNA表达，酶联免疫吸附测定（ELISA）法检测肝脏游离脂肪酸（FFA），脂肪组织AQP7和磷酸化p38MAPK（p-p38MAPK）的表达。结果：与正常组比较，模型组大鼠肝脏脂肪变性明显，血清TG，TC，AST，ALT和肝脏TG，FFA明显升高，脂肪组织中AQP7 mRNA及AQP7含量表达降低，p38MAPK mRNA及p-p38MAPK含量升高（P <0.05）；与模型组比较，祛痰活血颗粒和SB203580可减轻NAFLD大鼠肝脏脂肪变性程度；明显降低NAFLD大鼠的血清TG，TC，AST，ALT，肝脏TG，FFA（P <0.05）；各中药组和SB203580组大鼠脂肪组织AQP7 mRNA和AQP7含量表达明显升高，p38MAPK mRNA和p-p38MAPK含量明显降低（P <0.05）。结论：祛痰活血颗粒能减轻或者逆转肝脏的脂肪变性，其机制可能与抑制脂肪组织p38MAPK活化、上调AQP7表达，增加甘油入血代谢，减少进入肝脏的FFA，从而降低肝脏TG蓄积有关。

Abstract

Objective: To explore the mechanism of Qutan Huoxue granules in the treatment of non-alcoholic fatty liver disease (NAFLD) in rats. Method: The 40 SD rats were randomly divided into normal group (n=6) and model group (n=34). NAFLD rat models were induced by high fat diet

and after successful modeling

the successful modeling group (n=30) were further randomly divided into 5 groups

6 in each group:model group

Qutan Huoxue granules high

middle and low dose groups (10

2.5 g·kg-1) and SB203580 group (3 mg·kg-1). Intragastric administration of Qutan Huoxue granules and intraperitoneal injection of p38MAPK inhibitor SB203580 were given respectively for 4 weeks. Then the pathological changes of liver were observed by htoxylin-eosin(HE) staining and oil red O staining; Kits were used to detect the triglyceride (TG)

total cholesterol (TC)

aspartate transaminase (AST)

and alanine transferase (ALT) levels in serum

and TG levels in liver tissues; the adipose tissue aquaporin7 (AQP7) and p38 mitogen activated protein kinase (p38MAPK) mRNA expression levels were detected by Real-time PCR; enzyme linked immunosorbent assay(ELISA) was used to detect the expression of free fatty acid (FFA) in liver

AQP7 and phosphorylated p38MAPK (p-p38MAPK) expression in adipose tissues. Result: As compared with the normal group

the model group showed an obvious hepatic steatosis and a significant increase in serum TG

AST

ALT and liver TG

FFA levels

a significant decrease in the expression of AQP7 mRNA and AQP7 protein and an increase of p38MAPK mRNA and p-p38MAPK protein (P <0.05). As compared with the model group

Qutan Huoxue granules and SB203580 can reduce the degree of liver steatosis

and significantly decrease the serum TG

AST

ALT and liver TG

FFA levels in NAFLD rats (P <0.05). The Chinese medicine groups and SB203580 group showed a significant increase in the expression of AQP7 mRNA and AQP7 protein and a decrease of p38MAPK mRNA and p-p38MAPK protein in adipose (P <0.05). Conclusion: Qutan Huoxue granules can reduce or reverse the liver steatosis

and the mechanism may be related to inhibiting the activation of p38MAPK

up-regulating the expression of AQP7 in adipose tissues

increasing plasma glycerol metabolism

reducing glycerol and FFA intake into liver

hereby reducing TG accumulation in liver.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical Application and Mechanism of Sanrentang Based on Treating Different Diseases with Same Therapy： A Review

Effect and Mechanisms of Chinese Medicine and Its Active Ingredients in Enhancing Antibacterial Activities of Antibiotics： A Review

Mechanism of Fibrinogen Overexpression in Influencing Coronary Heart Disease with Syndrome of Blood Stasis in Rats Based on Mitochondrial Quality Control System

Pharmacodynamic Substances and Mechanisms of Da Chengqitang in Treating Stroke： A Review

Analysis on Pharmacodynamic Material Basis and Mechanism of Famous Classical Formula Renshen Wuweizi Tang in Treatment of Spleen and Lung Qi Deficiency Syndrome

Related Author

DOU Xin

PAN Haidi

HE Changhui

WEN Xiaoqing

MEI Xiao

MA Yuanxin

DENG Hualiang

WANG Wei

Related Institution

The First Clinical Medical College，Shandong University of Traditional Chinese Medicine（TCM）

College of TCM，Shandong University of TCM

The Affiliated Hospital of Shandong University of TCM

Jiangxi Provincial Key Laboratory of Natural and Biomimetic Drugs Research， School of Health， Jiangxi Normal University

Department of Medicine，China National Intellectual Property Administration

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰