Effect of Huashi Jiedu Decoction on Disease Outcome of Patients Infected with High-grade CIN and High-risk HPV with Shire Xiazhu Symptom After LEEP Operation

SUN Gui-xia; LI Yan-yun; YANG Shao-qin

doi:10.13422/j.cnki.syfjx.2017160182

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Effect of Huashi Jiedu Decoction on Disease Outcome of Patients Infected with High-grade CIN and High-risk HPV with Shire Xiazhu Symptom After LEEP Operation

更新时间：2024-01-04

- Effect of Huashi Jiedu Decoction on Disease Outcome of Patients Infected with High-grade CIN and High-risk HPV with Shire Xiazhu Symptom After LEEP Operation
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 16, Pages: 182-187(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017160182
  CLC： R737.33
- Published：2017
- 稿件说明：
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SUN Gui-xia, LI Yan-yun, YANG Shao-qin. Effect of Huashi Jiedu Decoction on Disease Outcome of Patients Infected with High-grade CIN and High-risk HPV with Shire Xiazhu Symptom After LEEP Operation[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 182-187.
DOI：

SUN Gui-xia, LI Yan-yun, YANG Shao-qin. Effect of Huashi Jiedu Decoction on Disease Outcome of Patients Infected with High-grade CIN and High-risk HPV with Shire Xiazhu Symptom After LEEP Operation[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(16): 182-187. DOI： 10.13422/j.cnki.syfjx.2017160182.

摘要

目的：探讨化湿解毒汤对高级别CIN高危型HPV感染者LEEP术后湿热下注证患者的临床疗效，并从凋亡抑制基因即生存素（Survivin）和增殖细胞核抗原（Ki67）表达及HPV病毒载量等方面探讨了其对转归的影响。方法：将160例CIN高危型HPV感染者，采用SAS软件生成，随机按1∶ 1比例分为观察组和对照组。两组患者均给予LEEP术治疗。术后对照组给予重组人干扰素α-2 b凝胶宫颈外涂，连续10 d为1个疗程，共使用3个月经周期。观察组在对照组治疗的基础上加用化湿解毒汤内服，1剂/d。连续服用3个月经周期，再行3个月经周期随访。检测术前和术后6个月患者Survivin，Ki67水平阳性表达情况和人乳头瘤病毒（HPV）DNA基因（HPV-DNA）病毒载量；进行术前和术后3个月慢性宫颈炎和湿热下注证的评价；检测术前和术后3个月宫颈局部灌洗液γ-干扰素（IFN-γ），白细胞介素-10（IL-10），白细胞介素-12（IL-12），白细胞介素-2（IL-2）和白细胞介素-4（IL-4）水平。结果：观察组临床疗效总有效率为94.52%，对照组为81.69%，观察组高于对照组（χ2=6.691，P <0.05）；治疗后观察组中医证候疗效总有效率为95.89%，对照组为78.87%，观察组高于对照组（χ2=9.529，P <0.01）；术后6个月，观察组高危型人乳头瘤病毒（hr-HPV）阴转率为91.78%，对照组为77.46%，观察组高于对照组（χ2=5.699，P <0.05）；观察组患者在术后6个月hr-HPV病毒载量低于对照组（P <0.01）；术后6个月，观察组Survivin和Ki67阳性表达情况弱于对照组（P <0.05），观察组Survivin阴转率为73.97%，高于对照组的56.34%（χ2=4.938，P <0.05），观察组Ki67阴转率为80.82%，高于对照组的59.15%（χ2=8.067，P <0.01）；术后3个月观察组IFN-γ，IL-2和IL-12水平均高于对照组，IL-4和IL-10水平均低于对照组（P <0.01）。结论：采用化湿解毒汤干预CIN高危型HPV感染LEEP术后患者，能提高患者的临床治疗效果，能促使hr-HPV阴转，降低病毒载量，能降低Survivin和Ki67阳性表达，并调节Th1/Th2平衡，从而起到控制CIN向宫颈癌转变，改善其预后。

Abstract

Objective: To discuss the clinical efficacy of Huashi Jiedu decoction on patients infected with high-grade cervical intraepithelial neoplasia (CIN) and high-risk human papillomavirus(HPV) Shire Xiazhu symptom after loop electrosurgical excision procedure(LEEP) operation

and the effect on disease outcome according to the expressions of apoptosis inhibition gene surviving and proliferating cell nuclear antigen Ki67 and the HPV viral load. Method: One hundred and sixty patients with high-grade cervical intraepithelial neoplasia CIN and high-risk HPV were randomly divided into control group (80 cases) and observation group (80 cases) according to random tables generated by SAS software. The two group's patients got LEEP operation. After operation

patients in control group were given LEEP operation recombinant human interferon α-2 b gel outside of cervix. A course of treatment was 10 days

and the treatment shall last for 3 continued menstrual cycles. In addition to the therapy of control group

patients in observation group were also given Huashi Jiedu decoction

1 dose/day for 3 continued menstrual cycles. And follow-up visit continued for 3 menstrual cycles. Before the operation and at the 6th month after the operation

positive expressions of Survivin

Ki67 and human papilloma virus (HPV) DNA gene (HPV-DNA) virus were detected. At the 3th month before and after the operation

chronic cervicitis and Shirexiazhu symptom were evaluated

and levels of γ-interferon (IFN-γ)

and levels of interleukin-2

12 (IL-2

12) were detected. Result: The total clinical rate in observation group was 94.52%

which was higher than 81.69% in control group (χ2=6.691

P <0.05). The total rate of traditional Chinese medicine(TCM) syndrome in observation group was 95.89%

which was higher than 78.87% in control group (χ2=9.529

P <0.01). Six months later after the operation

negative rate of human papilloma virus (HPV) was 91.78%

which was higher than 77.76% in control group (χ2=5.699

P <0.05). The hr\|HPV viral load of observation group was lower than that of control group (P <0.01). And positive expressions of Survivin and Ki67 in observation group were lower than that in control group (P <0.05). The negative rate of Survivin in observation group was 73.97%

which was higher than 56.34% in control group (χ2=4.938

P <0.05)

the negative rate of Ki67 in observation group was 80.82%

which was higher than 59.15% in control group (χ2=8.067

P <0.01). After 3 months of the operation

levels of IFN-γ

IL-2 and IL-12 in observation group were higher than those in control group

and levels of IL-4 and IL-10 in observation group were lower than those in control group (P <0.01). Conclusion: Huashi Jiedu decoction can improve the clinical efficacy of patients infected with high-grade cervical intraepithelial neoplasia CIN and high-risk HPV

promote the negative conversion rate of hr\|HIV

decrease the rate of viral load and the positive expressions of Survivin and Ki67

regulate the balance of Th1/Th2

so as to prevent CIN from developing to cervical cancer and improve the prognosis.

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