Metabolites from Endophytic Fungus  of

GUO Qing-feng; CHEN Lin; MA Jing-wei; KANG Wen-yi

doi:10.13422/j.cnki.syfjx.2017170053

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Metabolites from Endophytic Fungus of

更新时间：2024-01-04

- Metabolites from Endophytic Fungus of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 17, Pages: 53-57(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017170053
  CLC： R284.1
- Published：2017
- 稿件说明：
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GUO Qing-feng, CHEN Lin, MA Jing-wei, et al. Metabolites from Endophytic Fungus of [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(17): 53-57.
DOI：

GUO Qing-feng, CHEN Lin, MA Jing-wei, et al. Metabolites from Endophytic Fungus of [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(17): 53-57. DOI： 10.13422/j.cnki.syfjx.2017170053.

摘要

目的：研究垂丝海棠内生真菌Penicillium oxalicum的代谢产物。方法：从垂丝海棠叶片中分离得到一株内生真菌CLP001，通过18S rDNA的ITS基因序列比对分析对其进行鉴定，并利用液体静置培养的方法得到发酵产物，以及利用硅胶，LH-20羟丙基葡聚糖凝胶，半制备HPLC等多种柱色谱技术对其分离纯化，并根据化合物的光谱数据和理化性质进行结构鉴定。结果：内生真菌CLP001与P.oxalicum的相似性达到99%，因此被鉴定为P.oxalicum CLP001，从内生真菌发酵产物中分离鉴定了10个化合物，分别为亚油酸甘油三酯（1），1，2-亚油酸甘油二酯（2），1-单亚油酸甘油酯（3），单乙酸甘油酯（4），棕榈酸（5），亚油酸（6），麦角甾醇（7），1-D-阿拉伯醇-单亚油酸酯（8），尿嘧啶（9），反-丁烯二酸（10）。结论：其中化合物1~4以及8，10 为首次从该属中分离得到。

Abstract

Objective: To study the metabolites from the endophytic fungus Penicillium oxalicum of Malus halliana. Method: An endophytic fungus CLP001 was separated from the leaves of M. halliana and identified through its 18SrDNA ITS sequence analysis.The fermentation products were obtained by liquid static culture method

and further isolated and purified by silica gel

Sephadex LH-20 column chromatograghy and preparative HPLC method

and their structures were identified through spectral data and physiochemical properties. Result: The endophytic fungus CLP001 was identified as P. oxalicum CLP001 on basis of their similarity of 99%.Ten compounds were isolated and identified as trilinolein (1)

2-dilinolein (2)

1-monolinolein (3)

monacetin (4)

palmitic acid (5)

linoeic acid (6)

ergosterol (7)

1-D-arabinitol-monolinoleate (8)

uracil (9)

and (E)-butenedioic acid (10). Conclusion: Among them

compounds 1 - 4

8 and 10 were isolated for the first time from this genus.

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