Objective: To study the disturbance behavior of rutin and flavonoids in celery on the B-Z oscillating system

and provide a new simple and rapid method for determining the content of rutin and flavonoids. Method: The typical B-Z chemical oscillating system was used with rutin and the extraction of flavonoids in celery samples asoscillating substrate and disturbance component. A certain amount of rutin aqueous solutions was added to the above oscillating system at different periods to investigate the disturbanceruleson the oscillating spectra and oscillatingparameters; and the change of potential (E) with time (t) was recorded by using B-Z oscillation experiment device. Result: Under optimal conditions (stirring speed of 500 r·min-1

reaction temperature of 37 ℃)

the rutin was added in the peak position

and the disturbance rules of different concentrations of rutin on oscillating parameters showed good linear relationship between turin mass concentration and variation of oscillation period (T) within the range of 8.0×10-3-2.6×10-2 g·L-1. The linear equation: ΔT(s) = 699.4 × [rutin content] + 4.5

with the regression coefficient 0.996 8 and detection limit of 1.0×10-7 g·L-1 (S/N=3). Based on the standard curve of rutin by B-Z oscillating system

the determined contents of flavonoids in celery were comparable with those in the determination by using UV spectrophotometry. Conclusion: A new determination method for flavonoids content by using chemical oscillation technique is established

with advantages of short time

simple operation and low cost.