Identification of Metabolites of Rutin in Rats by UPLC-Q-TOF/MS

WU Ming-jiang; WU Xiao-lei; ZHANG De-qin; DING Li-qin; QIU Feng; ZHANG Hong-min

doi:10.13422/j.cnki.syfjx.2017170091

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Identification of Metabolites of Rutin in Rats by UPLC-Q-TOF/MS

更新时间：2024-01-04

- Identification of Metabolites of Rutin in Rats by UPLC-Q-TOF/MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 17, Pages: 91-97(2017)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2017170091
  CLC： R285.5
- Published：2017
- 稿件说明：
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WU Ming-jiang, WU Xiao-lei, ZHANG De-qin, et al. Identification of Metabolites of Rutin in Rats by UPLC-Q-TOF/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(17): 91-97.
DOI：

WU Ming-jiang, WU Xiao-lei, ZHANG De-qin, et al. Identification of Metabolites of Rutin in Rats by UPLC-Q-TOF/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(17): 91-97. DOI： 10.13422/j.cnki.syfjx.2017170091.

摘要

目的：研究大鼠口服芦丁后血浆、尿液、粪便和胆汁中的代谢产物。方法：按50 mg · kg-1剂量对Wistar大鼠一次性灌胃芦丁，取不同时间点的血浆、尿液、粪便和胆汁，采用超高效液相色谱-四级杆-飞行时间串联质谱（UPLC-Q-TOF/MS）对各生物样品进行检测，分析样品中芦丁的代谢产物。流动相乙腈（A）-0.1%甲酸水溶液（B）梯度洗脱（0~10 min，5%~15%A；10~15 min，15%~60%A；15~18 min，60%~90%A；18~19 min，90%~5%A；19~22 min，5%A），流速0.4 mL · min-1，样品温度4 ℃；电喷雾离子源（ESI），负离子监测模式。结果：从大鼠血浆、尿液、粪便和胆汁中共鉴定出22个代谢产物，其中血浆中7个，尿液中19个，粪便中12个，胆汁中15个，代谢产物主要包括Ⅰ相的水解代谢产物和Ⅱ相的葡萄糖醛酸结合、硫酸结合及甲基化代谢产物。结论：芦丁在大鼠体内存在多种代谢途径，代谢产物主要包括去糖基化产物和苷元槲皮素进一步生物转化的代谢产物，为芦丁的体内代谢提供了重要信息。

Abstract

Objective: To study on the metabolites in plasma

urine

feces and bile samples of rats after oral administration of rutin. Method: After disposable intragastric administration of rutin at a dose of 50 mg · kg-1

samples of plasma

urine

feces and bile were collected at different time points

and then all the samples were analyzed by UPLC-Q-TOF/MS. Result: A total of 22 metabolites of rutin were identified

including 7 metabolites in plasma

19 metabolites in urine

12 metabolites in feces and 15 metabolites in bile.Phase Ⅰ metabolites of rutin were hydrolytic metabolites and phase Ⅱ metabolites mainly included glucuronidation

sulfation and methylation metabolites. Conclusion: Rutin exists a variety of metabolic pathways in rats.The metabolites mainly include deglycosylated products and metabolites of quercetin.This study provides important information on the metabolism of rutin

which will be helpful for the further development and utilization of rutin.

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