Objective: To study the effect of amyloid beta (Aβ) injury-based human brain microvascular endothelial cell culture solution on normal neuronal apoptosis and the intervention effect of Tongxinluo capsule

in order to investigate the protective effect of Tongxinluo on brain microvascular and brain neurons and its possible mechanism. Method: The experiment was divided into blank group

normal group

model group

Aβ injury group

Tongxinluo group

Huperzine A group. The blank group was normal neuron group

The normal group was normal human brain microvascular endothelial cells-cultured neuron group. In the other 4 groups

20 mol · L-1 Aβ was used to induce damage in brain microvascular endothelial cells. Among them

the early intervention was made for the Tongxinluo group (pretreatment with 400 g · mL-1 Tongxinluo for 6 h) and the huperzine A group (pretreatment with 100 g · mL-1 huperzine A for 6 h). The damaged cell culture fluid was cultured in normal brain neurons. At the end of experiment

neuron apoptosis and apoptosis protein and gene expressions were detected. Result: The culture fluid of brain microvascular endothelial cells after Aβ damage could increase apoptosis rate of neurons in the brain

and directly cause severer cerebral neuron apoptosis than Aβ. After the early intervention with Tongxinluo

human brain microvascular endothelial cells-induced cerebral neuron apoptosis rate decreased

and apoptosis gene and protein expressions reduced (P<0.05

P<0.01)

with a better effect than western medicine huperzine A (P<0.05). Conclusion: The apoptosis of neurons could be further accelerated in Alzheimer's disease(AD)

and Tongxinluo protects brain microvascular endothelial cells and brain neurons. This suggests that Tongxinluo capsule has a double protective effect on AD neurons in the brain and brain microvascular endothelial cells.