Objective: To study the effect and mechanism of Puerarin on gentamicin-induced acute kidney injury. Method: The acute kidney injury model was built with gentamicin in Wister rats and divided into the no injury control group (blank group)

the acute kidney injury model group (model group)

and different doses of Puerarin treatment groups (subcutaneous injection of 0.5

5

50 mg · kg-1 Puerarin). Hematoxylin-eosin(HE) staining was used to observe the morphological changes in renal tissues of rats in each group; superoxide dismutase (SOD) activity

malondialdehyde (MDA)

total antioxidant capacity (TAOC)

inducible nitric oxide synthase (iNOS) contents in kidney tissues of rats were detected in each group. Meanwhile

the changes of serum creatinine (SCr)

urinary creatinine (UCr)

blood urea nitrogen (SBUN) and urea urea nitrogen (UBUN) levels were determined in all groups. Changes in monocyte chemoattractant protein-1 (MCP-1)

tumor necrosis factor alpha (TNF-α) and regulated on activation normal T cell expressed and secreted (RANTES)

macrophage inflammatory protein-2 (MIP-2) and other inflammatory protein contents were tested by enzyme linked immunosorbent assay(ELISA). And changes in the kidney injury molecular-1 (KIM-1) expression in renal tissues were detected by Western blot. Result: Compared with the no injury control group

in the gentamicin-induced acute kidney injury group

renal tubular necrosis score were increased

SCr

UCr

SBUN and UBUN content increased

the activity of SOD

TAOC decreased

the content of MDA and iNOS activity significantly increased

and MCP-1

TNF-α

RANTES

MIP-2 contents and KIM-1 protein content increased significantly(P<0.05). In different concentrations of Puerarin groups

scores for renal tubular necrosis in rats with acute kidney injury were decreased gradually

SCr

UCr

SBUN and UBUN decreased

while SOD

TAOC activity increased

MDA content

and iNOS activity decreased

MCP-1

RANTES

TNF-α

MIP-2 contents and KIM-1 protein expression decreased gradually (P<0.05). Conclusion: Puerarin can significantly reduce renal tubular necrosis in acute kidney injury rats induced by gentamicin

enhance function and antioxidant capacity of kidney tissues

and inhibit inflammatory reaction and KIM-1 protein expression

so as to reduce acute renal injury induced by gentamicin.