Objective: To observe the effect of double treasure decoction on serum progesterone (P)

β-human chorionic gonadotropin (β-HCG)

interferon-γ(INF-γ)

interleukin (IL)-2

IL-4

IL-10 levels

as well as its clinical efficacy for threatened abortion

investigate its effect on endocrine and cytokines and explore its tocolysis mechanism from the angle of maternal-fetal immune regulation and endocrine. Method: This prospective

randomized controlled study was conducted on the patients in Gynecology Outpatient Department of Henan Province Hospital of Traditional Chinese Medicine from April 2013 to November 2013.A total of 140 threatened abortion patients were recruited for the study. The volunteers were screened before enrollment and randomly allocated into two groups: 70 cases of Chinese medicine group and 70 cases of western medicine group.Patients in Chinese medicine received oral administration of double treasure decoction

and patients in western medicine group received oral administration of progesterone and intramuscular injection of HCG. Method of chemiluminescence was used to detect the concentration of P and β-HCG; enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration levels of serum cytokines INF-γ

IL-2 and IL-4

IL-10. Result: The concentration levels of serum P and β-HCG in Chinese medicine group were significantly higher than those in western medicine group (P<0.05); as compared with western medicine group

the contents of serum cytokines INF-γ and IL-2 were decreased significantly

while the contents of IL-4 and IL-10 were increased significantly in Chinese medicine group(P<0.05). Conclusion: The double treasure decoction can significantly improve the endocrine hormone and the levels of cytokines in patients with threatened abortion

and greatly improve the success rate of preventing miscarriages

further demonstrating that Strengthen Kidney for Fetus-soothing is the major solution of traditional Chinese medicine in the treatment of threatened abortion.