Optimization of Main Preparation Technology of Shufeng Dingchuan Granules Based on Bronchial Asthma Model of Guinea Pigs

MI Hui-juan; WANG Yong-xiang; WANG Xiu-hai; LI Lu; WU Yun; WANG Zhen-zhong

doi:10.13422/j.cnki.syfjx.2017180007

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Optimization of Main Preparation Technology of Shufeng Dingchuan Granules Based on Bronchial Asthma Model of Guinea Pigs

更新时间：2024-01-04

- Optimization of Main Preparation Technology of Shufeng Dingchuan Granules Based on Bronchial Asthma Model of Guinea Pigs
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 18, Pages: 7-10(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017180007
  CLC： R283.6
- Published：2017
- 稿件说明：
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MI Hui-juan, WANG Yong-xiang, WANG Xiu-hai, et al. Optimization of Main Preparation Technology of Shufeng Dingchuan Granules Based on Bronchial Asthma Model of Guinea Pigs[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 7-10.
DOI：

MI Hui-juan, WANG Yong-xiang, WANG Xiu-hai, et al. Optimization of Main Preparation Technology of Shufeng Dingchuan Granules Based on Bronchial Asthma Model of Guinea Pigs[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 7-10. DOI： 10.13422/j.cnki.syfjx.2017180007.

摘要

目的：优选疏风定喘颗粒的主体工艺路线，为该制剂的后续研究提供基础数据。方法：采用卵清蛋白致豚鼠支气管哮喘模型。随机分为空白组，模型组，氨茶碱组，咳喘宁组和疏风定喘颗粒工艺1，2，3，4组，自造模第15天起，将豚鼠置引喘仪内，空白组恒压喷入生理盐水，其他组恒压喷入10%卵蛋白，任动物自行吸入15 min，每天2次，连续7 d。豚鼠灌胃给药与诱喘同步进行，每组于当天2次诱喘间隔时间内灌胃给药，每日1次，连续7 d，空白组与模型组均灌胃给予等量生理盐水。以豚鼠引喘潜伏期，哮喘发作期，血清白细胞介素-4（IL-4），肿瘤坏死因子-α（TNF-α），免疫球蛋白E（IgE）含量为指标，优选疏风定喘颗粒的主体工艺路线。结果：与模型组相比，工艺1~4组样品均可降低哮喘豚鼠血清IL-4，TNF-α，IgE含量，延长豚鼠引喘潜伏期和哮喘发作期，且均有显著性差异（P<0.01）；与咳喘宁组相比，工艺4样品可延长豚鼠引喘潜伏期和哮喘发作期，且有显著性差异（P<0.05）；与氨茶碱组相比，工艺4样品可延长豚鼠引喘潜伏期，且有显著性差异（P<0.05）。与工艺2组相比，工艺4组样品可显著延长引喘潜伏期（P<0.05）。结论：选择工艺4为主体工艺路线，后续可进行工艺参数优化以开发疏风定喘颗粒。

Abstract

Objective: To optimize main preparation technology of Shufeng Dingchuan granules.Method: Ovalbumin was used to prepare bronchial asthma model in guinea pigs.The guinea pigs were randomly divided into normal group

model group

aminophylline group

Kechuanning group

Shufeng Dingchuan granules routing 1

3 and 4 groups.From the 15th day

the guinea pigs were placed in the apparatus which can cause asthma.The normal group were spray with saline

the other groups sprayed with 10% ovalbumin for consecutively 7 days

2 times a day

15 min at a time.At the same time

the normal group and model group were orally given normal saline

and the other groups were orally given the drug during the middle time of 2 times induced asthma for consecutively 7 days.With the length of asthma incubation time and asthma episodes time

the contents of interleukin-4(IL-4)

tumor necrosis factor-α(TNF-α) and immunoglobulin E (IgE) as indexes to determine the main preparation technology of Shufeng Dingchuan granules.Result: Compared with the model group

routing 1

3 and 4 groups could reduce the contents of IL-4

TNF-α and IgE in asthma guinea pigs (P<0.01)

and delay the asthma incubation period and asthma episodes period (P<0.01).Compared with Kechuanning group

the routing 4 samples could delay the asthma incubation period and asthma episodes period (P<0.05).Compared with aminophylline group

the routing 4 samples could delay the asthma incubation period (P<0.05).Compared with the routing 2 group

the routing 4 group could delay the asthma incubation period (P<0.05).Conclusion: Routing 4 is selected as the main preparation technology of Shufeng Dingchuan granules to further optimize the technological parameters.

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Related Institution

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