Objective: To establish the method to determine 3 types of components of Sanzisan

organic acid (gallic acid

chlorogenic acid)

iridoid aglycones (gardenoside

genipingentiobioside

geniposide)

crocin (crocin-I

crocin-Ⅱ) by using quantitative analysis of multi-components by single marker (QAMS) and validate its feasibility.Method: Shimadzu C18 column (4.6 mm×250 mm

5 μm) was used with mobile phase consisting of acetonitrile-0.1% phosphoric acid for gradient elution at a flow rate of 1.0 mL · min-1. The column temperature was 30℃ and the detection wavelength was (0-15 min) 271 nm

(16-45 min) 240 nm

(46-83 min) 330 nm

(84-115 min) 240 nm

and (116-150 min) 440 nm. The relative correction factors (RCF) of other components were calculated with geniposide as the internal reference. Then the RCF durability was investigated with 2 different HPLC instruments and 3 columns. Meanwhile

content of 7 components was determined by conventional external standard method

and t test was used to evaluate the difference between these two methods.Result: The results determined by two methods were compared

without significant differences

so QAMS can replace external standard method.Conclusion: The QAMS method was suitable for the quality control of Sanzisan.