Regulatory Effect of PI3K/Akt/mTOR Signal on Autophagy in Idiopathic Pulmonary Fibrosis and Intervention Effect of Astragaloside

XU Chang-jun; WANG Peng-fei; LIU Yang; HUANG Ya-wei; YANG Chang-fu; ZHAO Zong-jiang

doi:10.13422/j.cnki.syfjx.2017180075

您当前的位置：

首页 >

文章列表页 >

Regulatory Effect of PI3K/Akt/mTOR Signal on Autophagy in Idiopathic Pulmonary Fibrosis and Intervention Effect of Astragaloside

更新时间：2024-01-04

- Regulatory Effect of PI3K/Akt/mTOR Signal on Autophagy in Idiopathic Pulmonary Fibrosis and Intervention Effect of Astragaloside
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 18, Pages: 75-82(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017180075
  CLC： R285.5
- Published：2017
- 稿件说明：
移动端阅览
XU Chang-jun, WANG Peng-fei, LIU Yang, et al. Regulatory Effect of PI3K/Akt/mTOR Signal on Autophagy in Idiopathic Pulmonary Fibrosis and Intervention Effect of Astragaloside[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 75-82.
DOI：

XU Chang-jun, WANG Peng-fei, LIU Yang, et al. Regulatory Effect of PI3K/Akt/mTOR Signal on Autophagy in Idiopathic Pulmonary Fibrosis and Intervention Effect of Astragaloside[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 75-82. DOI： 10.13422/j.cnki.syfjx.2017180075.

摘要

目的：研究黄芪甲苷对特发性肺纤维化自噬活性影响及其磷酯酰肌醇3-激酶（PI3K）/蛋白激酶B（Akt）/mTOR信号的调控作用，探讨黄芪的抗肺纤维化机制。方法： c57bl/6小鼠分为假手术组、模型组、黄芪甲苷低、中、高剂量组、泼尼松组。博莱霉素（BLM）10 mg · kg-1诱导建立肺纤维化动物模型，以黄芪甲苷低、中、高剂量（25，50，100 mg · kg-1）干预，分别于3，7，14，28 d取材，进行苏木素-伊红（HE）染色观察肺组织形态变化，马松（Masson）三色染色、羟脯氨酸测定观察胶原表达水平，免疫组化及蛋白质免疫印迹（Western bolt）分析自噬标记蛋白和PI3K/Akt/mTOR信号蛋白表达水平。结果：与空白组比较，模型组肺组织炎症反应明显、胶原蛋白显著增加（P<0.01），PI3K/Akt/mTOR信号增强，自噬活性下降，与模型组比较，黄芪甲苷中、高剂量明显抑制肺组织炎症反应，下调转化生长因子-β1（TGF-β1）（P<0.01）和胶原蛋白（P<0.05）表达，提高LC3Ⅱ/Ⅰ和（beclin-1）表达（P<0.05），减少p62积累（P<0.05），同时抑制PI3K，Akt，mTOR磷酸化水平（P<0.05），减少ɑ-平滑肌动蛋白（ɑ-SMA）而提高E-钙黏素（E-cadherin）表达（P<0.01）。结论：黄芪甲苷通过抑制肺组织炎性反应、下调TGF-β1表达，抑制PI3K/Akt/mTOR信号增强肺组织细胞自噬活性，阻止上皮间质转分化（EMT）过程。

Abstract

Objective: To study the regulatory effect of phosphatidylinasitol 3-kinase(PI3K)/Akt/mTOR signal on the autophagy in idiopathic pulmonary fibrosis and the intervention effect of astragaloside.Method: The c57bl/6 Mice induced by bleomycin(BLM) were given astragaloside with low (25 mg · kg-1)

medium (50 mg · kg-1) and high (100 mg · kg-1) dose by gavage. At 3

28 days

lung tissues were collected for the analysis by hematoxylin-eosin (HE)

Masson

immunohistochemical

and Western blot.Result: Astragaloside with medium dose and high dose significantly inhibited the inflammatory reaction of lung tissues

down-regulated expression of transforming growth factor-β1 (TGF-β1)

increased LC3Ⅱ/Ⅰand beclin-1 expressions

decreased p62

inhibited phosphorylation levels of PI3K

Akt and mTOR

and strengthened cell autophagy activity.Conclusion: Astragaloside stopped the epithelial mesenchymal transdifferentiation(EMT) process by inhibiting the inflammatory reaction of lung tissues and the expression of TGF-β1

strengthening the autophagy activity and inhibiting PI3K/Akt/mTOR signal.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Astragaloside Ⅳ on Autophagy and Pyroptosis in Mice Bleomycin-induced Idiopathic Pulmonary Fibrosis

Improved Learning and Memory in Sleep-deprived Mice by Anmeidan via Cellular Autophagy Regulation Through PI3K/Akt/mTOR Signaling Pathway

Shengxiantang Alleviates Idiopathic Pulmonary Fibrosis in Rats by Regulating Oxidative Stress to Inhibit PANoptosis

Effect of Yishen Huayu Prescription on Autophagy of Transdifferentiated TCMK-1 Cells Based on SIRT1/FoxO1 Pathway

Clinical Randomized Controlled Trial of Traditional Chinese Medicine Compound Shenlong Decoction Granules in Treatment of Idiopathic Pulmonary Fibrosis

Related Author

LIN Chang

HUANG Mu-han

HAN Jia

OU Jiang-qin

DING Qian

XU Chang-jun

LIU Huizhen

ZHANG Yini

Related Institution

School of Traditional Chinese Medicine， Hubei University of Chinese Medicine

Engineering Research Center of Traditional Chinese Medicine Protection Technology and New Product Development for the Elderly Brain Health， Ministry of Education， Hubei University of Chinese Medicine

School of Basic Medical Sciences， Hubei University of Chinese Medicine

School of Pharmaceutics， Hubei University of Chinese Medicine

Hubei Shizhen Laboratory

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰