Objective: The development of epithelial-mesenchymal transition (EMT) is one of the key factors for tumor metastasis

so the research on the phenomenon of EMT may be conducive to control gastric cancer metastasis. This paper is aimed to explore the intervention effect and mechanism of Ruanjian Sanjie (RJSJ) granules for the invasion and metastasis of gastric carcinoma SGC-7901 cells.Method: Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was used to observe the inhibitory effect of RJSJ on SGC-7901 cells proliferation; transwell assay was used to explore the inhibitory effect of RJSJ granules on the invasion of gastric carcinoma SGC-7901 cells; the mRNA and protein expression levels of E-cadherin

Zinc finger E-box binding homeobox 1 (Zeb1) and Zinc finger E-box binding homeobox 2 (Zeb2) were examined by Real-time PCR and Western blot respectively.Result: MTT experimental results showed that all different concentrations of RJSJ granules could inhibit the proliferation of gastric carcinoma SGC-7901 cells (P<0.05)

and the inhibitory effect was in a dose and time dependent manner. transwell assay showed that RJSJ granules (concentrations of 250

500

1 000 mg · L-1 respectively) could inhibit invasion of gastric carcinoma SGC-7901 cells(P<0.05). Real-time PCR showed that RJSJ granules could up-regulate the mRNA expression levels of E-cadherin and down-regulate the mRNA expression levels of Zeb1 and Zeb2; while Western blot showed that RJSJ granules could up-regulate the protein expression levels of E-cadherin and down-regulate the protein expression levels of Zeb1 and Zeb2(P<0.05).Conclusion: RJSJ granules could inhibit SGC-7901 cells proliferation; RJSJ granules had an inhibitory effect on SGC-7901 cells EMT

and the mechanism may be associated with down-regulating Zeb1 and Zeb2 mRNA and protein expression and up-regulating E-cadherin mRNA and protein expression.