Objective: To study the effect of Shuangyi prescription (SYP)

component herbs and active ingredients on insulin resistance model of skeletal muscle cells

and glycogen synthesis

hexokinase (HK) and pyruvate kinase (PK) activity of IR-HepG2 cells

in order to analyze the mechanism

and lay a foundation for its clinical application.Method: The insulin resistance model of skeletal muscle cells was established by high concentration of insulin

in order to observe the effect of low

medium and high-dose (30

120

480 μg · L-1) SYP

component herbs (Astragali Radix

Corni Fructus

Coptidis Rhizome

Mori Cortex

Puerariae Lobatae Radix

Eupatorii Herba)

and different active ingredients (astragaloside

calycosin-7-glucoside

ursolic acid

loganin

berberine

puerarin

1-deoxynojirimycin) on insulin resistance model of skeletal muscle cells. The IR-HepG2 cells model of high concentration of insulin was established in vitro

so as to study the effects of glycogen synthesis

HK and PK activities of IR-HepG2 cells of SYP

component herbs and active ingredients. Western blot was performed to detect protein expressions of tyrosine 4 phosphatase-1B(PTP-1B) and glucose transporters 4(GLUT4).Result: SYP

Astragali Radix

Corni Fructus

astragaloside

1-deoxynojirimycin

and puerarin could reduce glucose concentration in skeletal muscle cell supernatant (P<0.05)

with a better effect in SYP

Astragali Radix and puerarin groups with the dose of 480 mg · L-1. Compared with the model group

SYP

Astragali Radix and active ingredients could decrease the expression of PTP-1B

and increase the expression of GLUT-4.Compared with the model group

different concentrations of active ingredients and component herbs could increase glycogen synthesis and HK

PK activities of HepG2 cells(P<0.05

P<0.01); Astragaloside and Astragali Radix extracts had a better effect in increasing glycogen synthesis and HK activity

and SYP and puerarin had a better effect in increasing PK activity.Conclusion: SYP

component herbs and active ingredients could relieve the insulin resistance of skeletal muscle and HepG2 cells

and its action mechanism may be decrease in the expression of PTP-1B

and increase in the expression of GLUT4

glycogen synthesis and HK

PK activities.