Objective: To investigate the effect of Xuefu Zhuyu capsule on Sirt1 signal transduction pathway of ischemic cardiomyocytes in rats

and discuss its mechanism of action.Method: A total of 70 healthy adult female wistar rats were randomly divided into normal group

sham operation group (10 mL · kg-1 distilled water)

model group (10 mL · kg-1 distilled water)

Xuefu Zhuyu high dose group (0.03 g · kg-1)

Xuefu Zhuyu middle dose group (0.02 g · kg-1)

Xuefu Zhuyu low dose group (0.01 g · kg-1)

and L-NAME group (2 mg/rat

intraperitoneal injection). The mRNA expression levels of Sirt1

p53 and NF-κB in myocardium were detected by real-time fluorescence quantitative PCR.Result: As compared with the sham operation group

the expression level of Sirt1 was significantly reduced in the model group (P<0.05); the expression levels of p53 and NF-κB in myocardium were increased in model group(P<0.05). As compared with the model group

the expression level of Sirt1 in ischemic myocardium was increased in Xuefu Zhuyu high

middle and low dose groups (P<0.05); the expression levels of p53 and NF-κB in myocardium were decreased in Xuefu Zhuyu high dose group (P<0.05).Conclusion: Xuefu Zhuyu capsule can inhibit the expression of p53 and NF-κB genes by activating Sirt1 signaling pathway to exert its anti-aging effect and prolong the life span of ischemic cardiomyocytes.