Effect of ginkgo leaves extract on Apoptosis in High Glucose-induced Human Retinal Pigment Epithelial Cells

NAN Na; ZHAO Ping; ZHANG Rui

doi:10.13422/j.cnki.syfjx.2017180168

您当前的位置：

首页 >

文章列表页 >

Effect of ginkgo leaves extract on Apoptosis in High Glucose-induced Human Retinal Pigment Epithelial Cells

更新时间：2024-01-04

- Effect of ginkgo leaves extract on Apoptosis in High Glucose-induced Human Retinal Pigment Epithelial Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 18, Pages: 168-173(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017180168
  CLC： R285
- Published：2017
- 稿件说明：
移动端阅览
NAN Na, ZHAO Ping, ZHANG Rui. Effect of ginkgo leaves extract on Apoptosis in High Glucose-induced Human Retinal Pigment Epithelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 168-173.
DOI：

NAN Na, ZHAO Ping, ZHANG Rui. Effect of ginkgo leaves extract on Apoptosis in High Glucose-induced Human Retinal Pigment Epithelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 168-173. DOI： 10.13422/j.cnki.syfjx.2017180168.

摘要

目的：观察银杏叶提取物对高糖诱导的人视网膜色素上皮细胞ARPE-19细胞凋亡的影响并初步探讨可能机制。方法：体外培养ARPE-19细胞株，分为正常组（5.5 mmol · L-1葡萄糖），高糖组（30 mmol · L-1葡萄糖），银杏叶提取物组（30 mmol · L-1葡萄糖+12.5，25，50，100 mg · L-1银杏叶提取物）。噻唑蓝（MTT）比色法检测细胞活性；流式细胞术测定细胞凋亡率；采用实时荧光定量PCR（Real-time PCR）和蛋白免疫印迹法（Western blot）测定B细胞白血病/淋巴瘤相关抗原-2（Bcl-2），白血病/淋巴瘤相关抗原相关X蛋白（Bax），半胱氨酸天冬氨酸蛋白酶-3（Caspase-3），凋亡相关因子（Fas），Fas受体（FasL） mRNA和蛋白的表达。Western blot检测c-Jun氨基末端激酶（JNK）磷酸化及核JNK表达变化。结果：高糖组吸光度A明显低于正常组（P<0.05），12.5，25，50，100 mg · L-1银杏叶提取物组比高糖组A明显升高（P<0.05）；高糖组细胞凋亡率明显高于正常组，12.5，25，50，100 mg · L-1银杏叶提取物组比高糖组细胞凋亡率明显降低（P<0.05）；与正常组比较，高糖组Bax和Caspase-3，Fas和FasL表达升高（P<0.05），12.5，25，50，100 mg · L-1银杏叶提取物组能降低Bax和Caspase-3的表达，升高Bcl-2表达水平，降低Fas和FasL表达（P<0.05）；与正常组比较，高糖组JNK磷酸化及核JNK蛋白水平升高（P<0.05），12.5，25，50，100 mg · L-1银杏叶提取物降低JNK磷酸化及核JNK表达（P<0.05）。结论：银杏叶提取物能通过JNK通路，抑制细胞凋亡，改善糖尿病视网膜病变。

Abstract

Objective: To investigate the effect of ginkgo leaves extract on the apoptosis in high-glucose-induced human retinal pigment epithelial cells

in order to explore possible mechanisms.Method: In vitro

ARPE-19 cells was divided into normal control group (5.5 mmol · L-1 glucose)

high-glucose group(30 mmol · L-1 glucose) and ginkgo leaves extract group (30 mmol · L-1 glucose+12.5

100 mg · L-1 ginkgo leaves extract). MTT assay was used to detect cell viability. Flow cytometry was used to determine the apoptotic rate. Bcl-2

Bax

Caspase-3

Fas and FasL mRNA and protein expressions were measured by Real-time PCR and Western blot. Western blot was used to detect the phosphorylation of JNK and the expression of nuclear JNK.Result: A value of the high-glucose group was significantly lower than that of the control group. Those in 12.5

100 mg · L-1 ginkgo leaves extract groups were significantly higher than those of the high-glucose group (P<0.05). The apoptotic rate of the high-glucose group was significantly higher than that of the control group. Those in 12.5

100 mg · L-1 ginkgo leaves extract groups were significantly lower than those of the high-glucose group (P<0.05). Compared with the control group

expressions of Bax

Caspase-3

Fas and FasL in the high-glucose group were increased. And expressions of Bax

Caspase-3

Fas and FasL were decreased by 12.5

100 mg · L-1 ginkgo leaves extract (P<0.05). JNK phosphorylation and nuclear JNK protein were increased in the high-glucose group

compared with the control group; and JNK expression was decreased in 12.5

100 mg · L-1 ginkgo leaves extract groups (P<0.05).Conclusion: ginkgo leaves extract could alleviate diabetic retinopathy and inhibit apoptosis through the JNK pathway.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma Ameliorates Myocardial Ischemia-reperfusion Injury in Rats via FUNDC1-dependent Mitophagy

Sequential Administration of Dihuang Baoyuan Granules and Fuling Yunhua Granules for Teating Type 2 Diabetes Mellitus in Mice

Hei Xiaoyaosan Improves Learning and Memory Abilities in Alzheimer's Disease Rats by Regulating Cell Apoptosis

Mechanism of Gushining Granules in Attenuating Dexamethasone-induced Apoptosis of Bone Marrow Mesenchymal Stem Cells via Activating PI3K/Akt/Bad Signalling Pathway

Traditional Chinese Medicine Regulates JAK/STAT Signaling Pathway to Treat Colorectal Cancer： A Review

Related Author

WANG Zhe

WANG Guoliang

WANG Ziyi

HAO Qianying

HE Qingyong

MA Xiaojuan

YIN Huijun

XIE Huiyi

Related Institution

Guang'anmen Hospital， China Academy of Chinese Medical Sciences

Xiyuan Hospital， China Academy of Chinese Medical Sciences

Beijing University of Chinese Medicine

Wangjing Hospital of China Academy of Chinese Medical Sciences

The First Affiliated Hospital of Guangzhou University of Chinese Medicine， Guangdong Clinical Research Academy of Chinese Medicine， Guangdong Engineering Research Center of Commercialization of Medical Institution Preparations and Traditional Chinese Medicines， Guangdong Engineering Technology Research Center of Commercialization of Linnan Special Medical Institution Preparations

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰