IL-6 Gene Promoter Methylation in Patients with Blood Stasis Syndrome and Unstable Angina

CHEN Guang; HE Hao-qiang; LIU Yong-mei; WANG Li; WANG Jie

doi:10.13422/j.cnki.syfjx.2017190023

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IL-6 Gene Promoter Methylation in Patients with Blood Stasis Syndrome and Unstable Angina

更新时间：2024-01-04

- IL-6 Gene Promoter Methylation in Patients with Blood Stasis Syndrome and Unstable Angina
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 19, Pages: 23-27(2017)
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- DOI：10.13422/j.cnki.syfjx.2017190023
  CLC： R259
- Published：2017
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CHEN Guang, HE Hao-qiang, LIU Yong-mei, et al. IL-6 Gene Promoter Methylation in Patients with Blood Stasis Syndrome and Unstable Angina[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 23-27.
DOI：

CHEN Guang, HE Hao-qiang, LIU Yong-mei, et al. IL-6 Gene Promoter Methylation in Patients with Blood Stasis Syndrome and Unstable Angina[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 23-27. DOI： 10.13422/j.cnki.syfjx.2017190023.

摘要

目的：研究冠心病不稳定性心绞痛（unstable angina，UA）患者外周血细胞中白细胞介素-6（IL-6）基因启动子区甲基化状态与中医血瘀证的关系。方法：选取6例2015年2月-2015年5月中国中医科学院广安门医院心血管科住院或门诊UA血瘀证患者作为试验组，另选取6例中国中医科学院广安门医院心内科住院或门诊UA非血瘀证患者作为对照组，采用重亚硫酸盐处理检测两组IL-6基因启动子区甲基化状态并进行组间比较，分析血瘀证与基因启动子区甲基化相关性。结果： IL-6基因转录起始位点前-1 118 bp至-826 bp序列中7个位点CpG位点甲基化程度血瘀证组存在高于非血瘀证组的趋势，但差异尚无统计学意义。IL-6基因转录起始位点前-1 47 1 bp至-1 184 bp序列4个CpG位点甲基化程度血瘀证组与非血瘀证组之间的差异尚不明显。结论： UA血瘀证患者IL-6基因启动子甲基化水平存在高于非血瘀证组的趋势，一定程度反映了UA血瘀证本质。

Abstract

Objective: To investigate the correlation between blood stasis syndrome (BBS) and the interleukin-6 (IL-6) gene promoter methylation state in the unstable angina (UA) patients. Method: Six BBS in patients or out patients from the Department of Cardiology

Guang'anmen Hospital of China Academy of Chinese Medical Sciences were recruited from February to May in 2015 as the test group

while 6 non BBS in patients or out patients were recruited as control group. The IL-6 gene promoter methylation states were detected by bisulfite and compared between two groups to analyze the correlation between BBS and gene promoter methylation state. Result: The methylation degree of BBS group was potentially higher than that of non BBS group on 7 sites from -1 118 to -826 bp before the transcription initiation site of IL-6

but with no statistically difference between the two groups; and there was no significant difference in the methylation degree on 4 sites from -1 471 to -1 184 bp before the transcription initiation site of IL-6 between two groups. Conclusion: It could be inferred that the methylation states of BBS group were potentially higher than those of non BBS group on IL-6 promoter

in part reflecting the essence of BBS.

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