Objective: To compare the different effect of Rehmanniae Radix Praeparata processed by different methods on the chemical damage anemia in mice as model of blood deficiency. Method: Blood deficiency model were induced by phenylhydrazine hydrochloride and cyclophosphamide.The experimental animals were randomly divided into blank group

model group

Ejiao blood enriching oral liquid group

traditional wine stewing group and origin processing integration group.The administration volume was 0.02 mL·g-1

and dose of the latter three was 10 mL·kg-1

3.75

3.75 g·kg-1

respectively.Enriching blood effect of Rehmanniae Radix Praeparata processed by different methods was evaluated by taking hemogram

level of erythropoietin(EPO) in serum and organ index as indexes. Result: Compared with the model group

red blood cells (RBC) of each administration group increased significantly (P<0.01);white blood cells (WBC) and hemoglobin (HB) of origin processing integration group increased significantly (P<0.01)

EPO was gradually restored to normal level and significantly decreased (P<0.01);WBC was increased in the traditional wine stewing group (P<0.05)

while EPO (P<0.05) returned to normal levels;Thymus index of origin processing integration group was significantly increased (P<0.01) and the parameter in traditional wine stewing group was increased (P<0.05).Compared with the blank group

these indexes of model group were significantly different.Compared with the Ejiao blood enriching oral liquid group

HB (P<0.05) was significantly increased in the origin processing integration group.Compared with the traditional wine stewing group

HB of the origin processing integration group was significantly increased (P<0.01). Conclusion: Rehmanniae Radix Preparata processed by different methods has a certain blood enriching effect.Among them

the origin processing integration group is better than the traditional wine stewing group.