Objective: Total phenolics

total flavonoids

polysaccharide

arecoline

arecaidine and guvacine were selected as target compositions

and meanwhile

ultraviolet spectrophotometry and ultra-performance liquid chromatography-diode array detector (UPLC-PDA) were used and combined with the changes of appearance traits and moisture content to investigate the effects of different storage environments and packaging materials on the quality of Arecae Semen. Method: The methods including folin-phenol method

aluminum trichloride coloring method and phenol-sulfuric acid method were adopted

and the contents of total phenolics

total flavonoids and polysaccharides were detected with a wavelength of 775

510

489 nm

respectively. The samples were extracted by ultrasonic approach with trichloromethane-methanol (4:1) as extracted solvent. Then

the analysis was performed on a BEH HILIC column (2.1 mm×100 mm

2.7 μm) with acetonitrile-10 mmol·L-1 ammonium acetate solution as the mobile phase for gradient elution. Finally

the contents of three alkaloids in Arecae Semen were determined at 215 nm by the established UPLC-PDA method. Result: After storage for 1 year under different storage conditions and packaging materials

areca appearance color was deepened

moisture content was increased

and the contents of total phenolics

total flavonoids

polysaccharide and alkaloids were reduced with the increase of storage time. In addition

their contents were significantly affected by high temperature and high humidity (P<0.01)

normal temperature and humidity (P<0.01) and packaging materials of woven bags (P<0.01) and kraft paper (P<0.01)

while low temperature and humidity (P<0.05) and self-sealed bags (P<0.05) showed little effect on the content of target compositions. Conclusion: Arecae Semen is recommended to be stored in self-sealed bags under low temperature and low humidity conditions for longer storage time and quality.