Objective: To investigate the chemical constituents from caulis of Lonicera alberti and establish an HPLC method for simultaneously determining the contents of chlorogenic acid

3

5-O-dicaffeoylquinic acid and 4

5-O-dicaffeoylquinic acid in different pharmaceutical parts of L. alberti. Method: Chemical constituents were isolated and purified by various chromatographic techniques such as silica gel

reversed-phase silica gel

macroporous resin

and Sephadex LH-20 column chromatography. Their structures were elucidated by physicochemical properties and spectroscopic data. The chromatographic conditions were as follows:Phenomenex C18 column (4.6 mm×250 mm

5 μm)

with acetonitrile (A) and 0.2% phosphoric acid solution (B) as the mobile phase for gradient elution (0-13 min

12%A; 13-25 min

12%-22%A; 25-45 min

22%A). The flow rate was 1.0 mL·min-1

detection wavelength at 327 nm

column temperature at 30℃

and injection volume at 10 μL. Result: Ten compounds were separated from L. alberti

and identified as 3

7-dimethylquercetin (1)

3

3'-dimethylquercetin (2)

quercetin (3)

ursolic acid (4)

loganin (5)

chlorogenic acid (6)

quercetin 5-O-β-D-glucoside (7)

3

4-O-dicaffeoylquinic acid (8)

3

5-O-dicaffeoylquinic acid (9) and 4

5-O-dicaffeoylquinic acid (10). Compounds 6

9 and 10 achieved baseline separation and showed good linearity

and the average recoveries were 100.15%

99.68% and 99.52% respectively. The content of three compounds from L. alberti flower(3.99%

1.77%

2.33%) was more than that of leaves (2.62%

1.73%

0.90%) and caulis (0.74%

0.25%

0.15%). Conclusion: Compounds 1

2 and 7 were isolated from Lonicera genus for the first time

and other compounds were found from this plant for the first time. The content determination method was accurate

reliable and convenient

so it could be used for content determination of chlorogenic acid

3

5-dicaffeoylquinic acid and 4

5-dicaffeoylquinic acid in L. alberti.