Identification of Polygala Saponins and Their Metabolites  from Ethanol Extract of Kaixinsan

Reeju Maharjan; Chiranjivi Thapa; WANG Na; Ram Chaulagain; XUE Di; JIA Yong-ming; DENG Zhu-jun; HUANG Shu-ming; LIU Xue-wei

doi:10.13422/j.cnki.syfjx.2017190118

您当前的位置：

首页 >

文章列表页 >

Identification of Polygala Saponins and Their Metabolites from Ethanol Extract of Kaixinsan

更新时间：2024-01-04

- Identification of Polygala Saponins and Their Metabolites from Ethanol Extract of Kaixinsan
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 19, Pages: 118-123(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017190118
  CLC： R285.5
- Published：2017
- 稿件说明：
移动端阅览
Reeju Maharjan, Chiranjivi Thapa, WANG Na, et al. Identification of Polygala Saponins and Their Metabolites from Ethanol Extract of Kaixinsan[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 118-123.
DOI：

Reeju Maharjan, Chiranjivi Thapa, WANG Na, et al. Identification of Polygala Saponins and Their Metabolites from Ethanol Extract of Kaixinsan[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 118-123. DOI： 10.13422/j.cnki.syfjx.2017190118.

摘要

目的：鉴定开心散60%乙醇提取物（KXS-60% E）及其血中移行成分中的远志皂苷类成分，分析大鼠体内远志皂苷类成分的代谢产物。方法：应用UPLC-TOF-MS技术，分析TOF-MS/MS给出的二级离子信息，结合液质联用工作站MassLynx v4.1分析软件及现有的文献数据库，鉴定KXS-60% E中远志皂苷类成分、灌服KXS-60% E后血清中的远志皂苷类成分及其代谢产物。结果：鉴定了KXS-60% E中14个远志皂苷类成分，主要为三萜型皂苷；鉴定了大鼠口服KXS-60% E后血中的5个原型皂苷类成分及4个代谢产物。结论：远志皂苷类成分及4个代谢产物可能是KXS-60% E潜在的活性成分及直接药效物质基础，这将有助于阐明开心散防治阿尔兹海默症的有效成分及作用机制。

Abstract

Objective: Polygala saponins in the 60% ethanol extract of Kaixinsan (KXS-60% E) were identified

and the polygala saponins as well as their metabolites were analyzed in rat plasma after oral administration of KXS-60% E. Method: Ion fragments data of polygala saponins in KXS-60% E was identified by UPLC-TOF-MS.Similarly

the polygala saponins and their metabolites were also identified in rat plasma after oral administration of KXS-60% E with the ion fragments data

MassLynx v4.1 software and databases in the literatures. Result: A total of 14 polygala saponins which were mainly triterpene saponins

were identified in KXS-60% E in vitro.Moreover

5 polygala saponins as well as 4 metabolites were identified in rat plasma. Conclusion: Five polygala saponins as well as four metabolites may be the potential bioactive compounds and direct pharmcodynamic basis of KXS-60% E.Further study on these constituents may find out the effective compounds in KXS-60% E and clarify the mechanism on treatment of Alzheimer's disease.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

In Vitro and in vivo Component Identification of Danshenyin Based on UPLC-Q-TOF-MS/MS

Identification of Prototype Compounds and Their Metabolites in Rat Plasma After Oral Administration of Aurantii Fructus Extract by UPLC-Q-TOF/MS

HPLC Fingerprint Analysis of Polygalae Radix with Different Commercial Grades

Mechanism of Different Dosage Forms of Kaixinsan in Improving Mitochondrial Function for Prevention and Treatment of Cognitive Disorder Based on AMPK/PGC-1α/SIRT3 Pathway

Related Author

ZHANG Sitong

HU Xianrun

LIU Wenkang

LEI Jinchun

CHENG Xuemei

WU Xiaojun

CHEN Wansheng

LI Manlin

Related Institution

Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine

（Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM），Ministry of Education，School of Basic Medical Sciences，Jiangxi University of TCM，，）

College of Traditional Chinese Medicine and Health Care， Guangdong Food and Drug Vocational College

Science and Technology Innovation Center，Guangzhou University of Chinese Medicine

Liaoning University of Traditional Chinese Medicine（TCM）

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰