HU Yuan-yuan, LYU Rui-hong, JI Yong-sheng, et al. Investigation of Deproteinization of Astragalus Polysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 25-29.
DOI:
HU Yuan-yuan, LYU Rui-hong, JI Yong-sheng, et al. Investigation of Deproteinization of Astragalus Polysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 25-29. DOI: 10.13422/j.cnki.syfjx.2017200025.
Investigation of Deproteinization of Astragalus Polysaccharide
Objective: Astragalus polysaccharide is an important active ingredient with the immune regulation
anti-oxidation
anti-cancer
anti-atherosclerosis and so on.To remove the proteins from astragalus polysaccharide is a critical procedure
because the content of protein in astragalus polysaccharide was higher than 15%.In this work
the purity would be improved by removing the proteins from astragalus polysaccharide
which can be in favor of the study on pharmacological activity and mechanisms of this effective site. Method: Astragalus polysaccharide was prepared by the water-extraction and alcohol-precipitation method
and the proteins in astragalus polysaccharide were removed by enzymatic-Sevage process
then the technolology was optimized with orthogonal test and single factor tests
respectively.Optimum technology was selected based on the purity of astragalus polysaccharide and the content of proteins. Result: Optimum process of deproteinization was as follows:the enzyme-substrate ratio of 2.0%
pH of 5.0
hydrolysis for 24 h at 50℃;under these conditions
the removal rate of proteins and the content of astragalus polysaccharide were 87.80% and 83.47%
respectively. Conclusion: This optimal technology is stable and feasible
enzymatic-Sevage method can significantly improve the purity of astragalus polysaccharide.
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Related Institution
Modern Research Center for Traditional Chinese Medicine(TCM),Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM,Shanxi University
Institute of Process Engineering,Chinese Academy of Sciences
Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine