Simultaneous Determination of Six Components in Bushen Qiangshen Pills

LIN Xiu-lian; SONG Fen-yun; LI Hua; CHEN Yu-xuan; ZHOU Ying-yi

doi:10.13422/j.cnki.syfjx.2017200056

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Simultaneous Determination of Six Components in Bushen Qiangshen Pills

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- Simultaneous Determination of Six Components in Bushen Qiangshen Pills
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 20, Pages: 56-60(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017200056
  CLC： O657.72;R286.0
- Published：2017
- 稿件说明：
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LIN Xiu-lian, SONG Fen-yun, LI Hua, et al. Simultaneous Determination of Six Components in Bushen Qiangshen Pills[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 56-60.
DOI：

LIN Xiu-lian, SONG Fen-yun, LI Hua, et al. Simultaneous Determination of Six Components in Bushen Qiangshen Pills[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 56-60. DOI： 10.13422/j.cnki.syfjx.2017200056.

摘要

目的：采用超高效液相色谱法（UPLC）同时测定补肾强身片中原儿茶酸、绿原酸、金丝桃苷、特女贞苷、朝藿定C和淫羊藿苷的含量。方法：以Agilent SB-C18色谱柱（4.6 mm×100 mm，2.7 μm），乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱，流速0.5 mL ·min-1，柱温40℃，检测波长260，325，360，224，270 nm。结果： 6个成分的检测范围分别为原儿茶酸0.78~201.00 mg ·L-1（r=0.999 9），绿原酸0.79~202.60 mg ·L-1（r=0.999 7），金丝桃苷0.93~238.39 mg ·L-1（r=0.999 9），特女贞苷0.66~169.62 mg ·L-1（r=0.999 9），朝藿定C 0.81~206.85 mg ·L-1（r=0.999 9），淫羊藿苷0.74~190.85 mg ·L-1（r=0.999 9）。平均回收率分别为 97.6%（RSD 1.0%），99.6%（RSD 1.5%），99.7%（RSD 1.5%），97.2%（RSD 0.8%），95.5%（RSD 0.4%），96.3%（RSD 0.9%）。结论：该方法简便可靠，可用于补肾强身片的质量控制。

Abstract

Objective: To simultaneously determinate the contents of protocatechuic acid

chlorogenic acid

hyperoside

specnuezhenide

epmedin C and icariin in Bushen Qiangshen pills (BQP) by developing an Ultra high performance liquid method(UPLC). Method: The UPLC separation was performed on an Agilent SB-C18 reversed-phase column (4.6 mm×100 mm

2.7 μm) with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution.The detective wavelength was set at 260

325

360

224

270 nm. Result: The calibration curve was linear when amount was within 0.78-201.00 mg ·L-1 for protocatechuic acid (r=0.999 9); 0.79-202.60 mg · L-1 for chlorogenic acid (r=0.999 7); 0.93-238.39 mg ·L-1 for hyperoside (r=0.999 9); 0.66-169.62 mg ·L-1 for specnuezhenide (r=0.999 9); 0.81-206.85 mg ·L-1 for epmedin C (r=0.999 9); and 0.74-190.85 mg ·L-1for icariin (r=0.999 9).The recoveries were 97.6%

99.6%

99.7%

97.2%

95.5%

96.3% with RSDs of 1.0%

1.5%

0.8%

0.4% and 0.9%

respectively. Conclusion: This simple and reliable method can be used for the quality control of BQP.

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