Simultaneous Determination of Six Main Active Ingredients in Jiawei Xiangru Oral Liquid by HPLC

LI Ke; LU Mao-fang; CHEN Dan; HOU Xi; LI Ruo-cun

doi:10.13422/j.cnki.syfjx.2017200061

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Simultaneous Determination of Six Main Active Ingredients in Jiawei Xiangru Oral Liquid by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Six Main Active Ingredients in Jiawei Xiangru Oral Liquid by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 20, Pages: 61-65(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017200061
  CLC： O657.72;R286.0
- Published：2017
- 稿件说明：
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LI Ke, LU Mao-fang, CHEN Dan, et al. Simultaneous Determination of Six Main Active Ingredients in Jiawei Xiangru Oral Liquid by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 61-65.
DOI：

LI Ke, LU Mao-fang, CHEN Dan, et al. Simultaneous Determination of Six Main Active Ingredients in Jiawei Xiangru Oral Liquid by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 61-65. DOI： 10.13422/j.cnki.syfjx.2017200061.

摘要

目的：建立同时测定加味香薷口服液（葛根、香薷、厚朴、陈皮、蚕沙、白扁豆）中葛根素、麝香草酚、香荆芥酚、和厚朴酚、橙皮苷、大豆苷含量的方法。方法：采用高效液相色谱法。采用Hypersil C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.01%冰乙酸溶液（梯度洗脱），流速1 mL · min-1，检测波长277 nm，柱温30℃。结果：葛根素、麝香草酚、香荆芥酚、和厚朴酚、橙皮苷、大豆苷分别在0.384~4.80 μg（r=0.999 3），0.048~0.60 μg（r=0.999 7），0.015 6~0.195 μg（r=0.999 8），0.018 2~0.227 5 μg（r=0.999 2），0.226~2.825 μg（r=0.999 6），0.228~2.850 μg（r=0.999 5）进样量与峰面积呈良好的线性关系；平均加样回收率分别是葛根素97.98%，RSD 2.1%，麝香草酚98.88%，RSD 1.8%，香荆芥酚98.10%，RSD 2.1%，和厚朴酚100.12%，RSD 1.5%，橙皮苷100.40%，RSD 2.6%，大豆苷96.70%，RSD 1.6%。结论：该方法操作简便、稳定、重复性好，可用于加味香薷口服液中葛根素、麝香草酚、香荆芥酚、和厚朴酚、橙皮苷、大豆苷含量的同时测定。

Abstract

Objective: To establish a method for simultaneously determining the contents of puerarin

thymol

carvacrol

honokiol

hesperidin and daidzin in Jiawei Xiangru oral liquid (Puerariae Lobatae Radix

Moslae Herba

Magnoliae Officinalis Cortex

Citri Reticulatae Pericarpium

Bombyx Mori Linnacus and Lablab Semen Alabum). Method: High performance liquid chromatography (HPLC) was performed on Hypersil C18 chromatographic column (4.6 mm×250 mm

5 μm) with mobile phase of acetonitrile-0.01% glacial acetic acid (gradient elution) at a flow rate of 1 mL · min-1.The detction wavelength was 277 nm

and the column temperature was 30℃. Result: Puerarin

thymol

carvacrol

honokiol

hesperidin and daidzin showed good linear relationship between injection volume and peak area within the range of 0.384-4.800 μg (r=0.999 3)

0.048-0.600 μg (r=0.999 7)

0.015 6-0.195 0 μg (r=0.999 8)

0.018 2-0.227 5 μg (r=0.999 2)

0.226-2.825 μg (r=0.999 6)

and 0.228-2.850 μg (r=0.999 5)

respectively.The average recovery rate was 97.98%

98.88%

98.10%

100.12%

100.40% and 96.70%

respectively for puerarin

thymol

carvacrol

honokiol

hesperidin and daidzin

with RSDs of 2.1%

1.8%

2.1%

1.5%

2.6%

and 1.6%. Conclusion: The method is simple

stable and reproducible

and it can be used for the simultaneous determination of puerarin

thymol

carvacrol

honokiol

hesperidin and daidzin contents in Jiawei Xiangru oral liquid.

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Related Author

ZENG Hui-fang1

ZENG Bao2

HUANG Yao-hai2

SU Zi-ren2

Yi-ming KONG

Hui-jie YAN

Feng-qian GUO

Xin-jian LI

Related Institution

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Chengde Jingfukang Pharmaceutical Group Co.,Ltd.

Hebei Provincial Engineering Technology Research Center for New Pharmaceutical Excipients in Traditional Chinese Medicine

Guangdong Pharmaceutical University

Nanning institute for Food and Drug Control of Guangxi

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