CHEN Ke-ke, QIANG Yi. GC-MS Analysis of Fat-soluble Components from Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 77-81.
DOI:
CHEN Ke-ke, QIANG Yi. GC-MS Analysis of Fat-soluble Components from Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 77-81. DOI: 10.13422/j.cnki.syfjx.2017200077.
GC-MS Analysis of Fat-soluble Components from Different Parts of
目的: 研究和比较费菜根、茎、叶和果实4个部位的脂溶性成分。方法: 采用索氏提取法提取费菜根、茎、叶和果实4个部位的脂溶性成分,采用氢氧化钾-甲醇直接酯化法进行甲酯化处理,采用气相色谱-质谱联用技术(GC-MS)对4种脂溶性成分进行分离和鉴定。载气为高纯度氦气,进口温度250℃,流速1.24 mL · min-1;电离方式EI离子源,离子源温度200℃,接口温度250℃,溶剂延时3.5 min,m/z 40~600。结果: 从费菜的根、茎、叶和果实4个部位中分别鉴定出26,37,37,20种脂溶性成分,4个部位中共有的脂溶性成分有12种,单独存在的成分分别有6,6,9,2种。以亚麻酸和亚油酸为主的不饱和脂肪酸是费菜的主要脂溶性成分,其在根、茎、叶和果实4个部位的质量分数分别为42.59%,52.57%,37.04%和80.06%,4个部位的不饱和脂肪酸和饱和脂肪酸的比例分别为1.17:1,1.38:1,0.85:1和4.56:1,费菜的4个部位具有相似的脂肪酸组成,但脂肪酸的构成比例有一定差异。结论: 该文提取、鉴定并比较了费菜4个部位的脂溶性成分,为费菜的开发利用奠定了基础。
Abstract
Objective: To study and compare the fat-soluble components from the roots
stems
leaves and fruits of Sedum aizoon. Method: The fat-soluble components from different parts of S.aizoon were extracted by soxlet extraction.The fat-soluble components were esterified by using potassium hydroxide-methanol direct esterification method and then analyzed by gas chromatography-mass spectrometry (GC-MS).Carrier gas was high-purity helium; sample injection temperature was 250℃; velocity of flow was 1.24 mL · min-1; ionization mode was EI ion source
with ion source temperature of 200℃
interface temperature of 250℃
solvent delay of 3.5 min
m/z 40-600. Result: The 26
37
37 and 20 kinds of fat-soluble components were identified from roots
stems
leaves and fruits respectively.12 kinds of them were contained in all of the four parts
while 6
6
9 and 2 kinds were only present in roots
stems
leaves or fruits respectively.The main components of fat-soluble components were unsaturated fatty acids
which were mainly composed of linolenic acid and linoleic acid.The contents of unsaturated fatty acids were 42.59%
52.57%
37.04% and 80.06% respectively in roots
stems
leaves and fruits.The ratios of unsaturated fatty acids to saturated fatty acids in the different parts were 1.17:1
1.38:1
0.85:1 and 4.56:1.The four parts had similar fatty acid compositions
but there were some differences in the composition ratios. Conclusion: The fat-soluble components of S.aizoon were extracted
identified and compared for the first time.This research could establish foundation for further research and development of S.aizoon.