Objective: To explore the effect of Scrophulariae Radix on rat model with dampness stagnancy due to spleen deficiency

and to analyze biomarkers and metabolic pathways based on liver metabonomics. Method: The high fat and low protein diet plus swimming for 8 weeks under weight bearing condition was adopted to establish rat model with dampness stagnancy due to spleen deficiency.Liver index

serum albumin

blood fat

gastrointestinal function and water load were measured at 2 weeks after administration of high(5.40 g · kg-1) and low(1.35 g · kg-1) dose of Scrophulariae Radix decoction.UPLC-Q-TOF-MS was employed to analyze metabolites in liver tissue under electrospray ionization positive and negative ion mode with m/z 100-1 500.Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were adopted to compare the differential metabolites among the normal group

the model group

the high and low dose group of Scrophulariae Radix decoction;these different metabolites among four groups were analyzed by KEGG metabolic pathway method. Result: Compared with the model group

gastric emptying rate and D-xylose excretory rate in the high dose group were increased significantly;contents of serum albumin and total protein in the low dose group were increased;contents of low density lipoprotein-cholesterol

total cholesterol and water load index in the high dose group were decreased.A total of 21 biomarkers were identified and 7 major metabolic pathways were found

which mainly related to the insulin secretion

purine metabolism

bile acid secretion and so on. Conclusion: Treatment of Scrophulariae Radix on rat model with dampness stagnancy due to spleen deficiency is connected with improving the digestive function

inhibition of glucose metabolism

improving blood lipid metabolism and so on.