SUN Lei, FENG Jun, LEI Yan-li, et al. Determination of Equilibrium Solubility and Oil-water Partition Coefficient of Baicalin-berberine Hydrochloride Complex[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 35-38.
DOI:
SUN Lei, FENG Jun, LEI Yan-li, et al. Determination of Equilibrium Solubility and Oil-water Partition Coefficient of Baicalin-berberine Hydrochloride Complex[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 35-38. DOI: 10.13422/j.cnki.syfjx.2017210035.
Determination of Equilibrium Solubility and Oil-water Partition Coefficient of Baicalin-berberine Hydrochloride Complex
Objective: To determine the equilibrium solubility and the oil-water partition coefficient of baicalin-berberine hydrochloride complex for further study on the related preparations. Method: HPLC was validated for analysis of berberine hydrochloride and baicalin.The equilibrium solubilities of baicalin-berberine hydrochloride complex in water and media with different pH were measured by shaking flask method.The concentration of this complex in n-octanol and water was determined to calculate the oil-water partition coefficient. Result: The solubilities of berberine hydrochloride and baicalin in the complex were 2-fold and 50-fold lower than that of crude drugs in medium with pH of 6.8-8.0.The oil-water partition coefficients of baicalin and berberine hydrochloride in the complex were 2.37 times and 4.69 times higher than that of the pure drugs
respectively. Conclusion: Differences of solubility and partition coefficient are revealed between baicalin-berberine hydrochloride complex and pure drugs
which may affect the in vivo performance and gastrointestinal absorption of the complex.
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