Objective: To investigate the anti-complement constituents from the fruits of Toona sinensis. Method: The anti-complement constituents were screened by hemolysis method

then isolated and purified with different chromatographic techniques.Their structures were elucidated with the methods of 1H-NMR

13C-NMR and together with the comparison of reported data. Result: The methanol extracts of the fruits of T.sinensis showed apparent anti-complement activity

and fourteen polypenols were isolated from the active EtOAc fraction.The structures of the compounds were identified as gallic acid (1)

methyl gallate (2)

6-O-galloyl-D-glucpyranose (3)

1

2

3-tri-O-galloyl-β-D-glucopyranose (4)

1

2

6-tri-O-galloyl-β-D-glucopyranose (5)

1

2

3

6-tetra-O-galloyl-β-D-glucopyranose (6)

1

2

3

4

6-penta-O-galloyl-β-D-glucopyranose (7)

quercetin-3-O-α-L-rhamnopyanoside (8)

rutin (9)

isorhamnetin-3-O-β-galactopyranoside (10)

quercetin-3-O-β-galactopyranoside (11)

kaempferol-3-O-β-D-glucopyranoside (12)

kaempferol-3-O-α-L-rhamnopyranoside (13)

and quercetin (14).Compounds 3-14 were isolated from the fruits of T.sinensis for the first time

and compounds 5

10 and 11 were isolated for the first time from the plant.Compounds 4-7 showed apparent anti-complement activity

with the IC50 of 88.3

76.2

13.9 and 9.6 μmmol·L-1 respectively. Conclusion: The methanol extracts of the fruits of T.sinensis showed apparent anti-complement activity

and the EtOAc fraction was the active fraction.Epigallocatechin glucoside derivatives and flavonoids were the active components

and the former ones showed more potent anti-complement activity than the flavonoids; in addition

the activity was increased with the increasing of numbers of galloyl moieties.