Contents of Four Diterpenoids and Cytotoxic Activity in

HU Hai-tao; LIU Ya-lin; TIAN Shuang-shuang; ZHANG Ling-xia; CHEN Sui-qing; WU Hong; LIU Rui-xin; DAI Li-ping

doi:10.13422/j.cnki.syfjx.2017210059

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Contents of Four Diterpenoids and Cytotoxic Activity in

更新时间：2024-01-04

- Contents of Four Diterpenoids and Cytotoxic Activity in
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 21, Pages: 59-63(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017210059
  CLC： R285;R284.1
- Published：2017
- 稿件说明：
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HU Hai-tao, LIU Ya-lin, TIAN Shuang-shuang, et al. Contents of Four Diterpenoids and Cytotoxic Activity in [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 59-63.
DOI：

HU Hai-tao, LIU Ya-lin, TIAN Shuang-shuang, et al. Contents of Four Diterpenoids and Cytotoxic Activity in [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 59-63. DOI： 10.13422/j.cnki.syfjx.2017210059.

摘要

目的：以冬凌草甲素，毛叶香茶菜素 E，毛栲利素，香茶菜素 B为指标，建立HPLC同时测定鄂西香茶菜中4个二萜的质量控制方法，并对4个成分进行体外抗肿瘤活性的评价。方法：采用YMC C18反相色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-水（38：62），流速1.0 mL·min-1，柱温30 ℃，检测波长230 nm；采用噻唑蓝（MTT）比色法测定了鄂西香茶菜中4个指标性成分对HCT-116，HepG2，BGC-823，MB-231，A2780细胞的增殖抑制作用。结果：冬凌草甲素，毛叶香茶菜素 E，毛栲利素，香茶菜素 B分别在0.085 6~0.856，0.014 04~0.140 4，0.070 8~0.708，0.018 98~0.189 8 g·L-1呈良好的线性关系，平均回收率分别为101.78%，101.70%，100.23%，104.60%。4个二萜均显示了一定的细胞增殖抑制作用，其中冬凌草甲素，毛栲利素对5种细胞株的增值抑制率均>50%。冬凌草甲素、毛栲利素对5株肿瘤细胞株的细胞毒作用均优于毛叶香茶菜素 E和香茶菜素 B。半数抑制浓度（IC50）分别为4.10~6.46，2.34~4.77 μmol·L-1。结论：该方法简便，灵敏，重复性好，可用于鄂西香茶菜中主要细胞增殖抑制二萜的质量控制。

Abstract

Objective: To establish a method for simultaneous determination of four diterpenoids in Isodon henryi

and evaluate the in vitro antitumor activity of four diterpenes (oridonin

zea pollinium

lasiokaurin

rabdosin B). Method: YMC C18 column (4.6 mm × 250 mm

5 μm) was adopted with acetonitrile-water(38:62) as mobile phase for isocratic elution. The flow rate was 1.0 mL·min-1;the column temperature was 30 ℃; and the detection wavelength was at 230 nm. They were tested for their cytotoxic effects against five human cancer cell lines: HCT-116

HepG2

BGC-823

MB-231

and A2780 by MTT. Result: A good linearity was observed in the range of 0.085 6-0.856

0.014 04-0.140 4

0.070 8-0.708

0.018 98-0.189 8 g·L-1 for oridonin

maoyecrystal E

lasiokaurin

rabdosin B

with the average recoveries of 101.78%

101.70%

100.23%

104.60%

respectively; 4 diterpenes showed a certain cytotoxic effect

in which oridonin

lasiokaurin 5 kinds of cell strains of the rate of inhibition were greater than 50%. Oridonin

lasiokaurin exhibited more obviously cytotoxic activity in the tested cell lines with IC50 values ranging from 4.10-6.46

2.34-4.77 μmmol·L-1 respectively. Conclusion: The method is simple

sensitive and reproducible

and it can be used for the quality control of the main cytotoxic active diterpenes in I.henryi.

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