Objective: To develop a method of HPLC fingerprinting analysis for the quality evaluation of Swertiae Herba. Method: The Waters 2695-2996

Dionex Ultimate 3000 and Agilent-1260 high performance liquid chromatograph instrument equipped with Shiseido CAPCELL PAK C18 MGⅡ(4.6 mm×250 mm

5 μm)

Boston Green ODS (4.6 mm×250 mm

5 μm) and Phenomenex Gemini C18 110A column (4.6 mm×250 mm

5 μm) was used. Methanol- acetonitrile-0.05 mol·L-1 sodium dihydrogen phosphate solution (adjusted pH value to 3.9 with 0.1% phosphoric acid solution)(15:5:80) were used as mobile phase;the wavelength was set at 268 nm

and the flow rate was 1.0 mL·min-1. The principal component relative retention time comparison method was used to evaluate the characteristic fingerprints of 10 batches of Swertiae Herba produced in different regions. Result: The chromatographic fingerprint was completed with five common peaks

and the RSD of relative retention time for the common peaks from 10 batches of Swertiae Herba was less than 2.0%. Conclusion: The method is simple

accurate and sensitive

which can be used for the quality control of Swertiae Herba.