Objective: To research the resistance effect of radix of Euphorbia fisheriana(REF) combined with Jujubae Fructus(JF) on liver cirrhosis ascites in rat model. Method: Phenobarbital combined with CCl4 method was used to establish the rat live cirrhosis ascites model.The rats were divided into 4 groups

namely normal group

model group

REF group

and REF combined with JF group

with 10 rats in each group.Water metabolism and liver pathological changes in 4 groups were observed by HE staining method.Expressions of Collagen Ⅰ

Collagen Ⅲ

Fibronectin (FN)

Laminin

Desmin in different groups of rat liver issues were determined by immunohistochemical method. Result: Compared with the model group

the REF group and the REF combined with JF group significantly increase 24 h urine volume (P < 0.01)

and reduced peritoneal seroperitoneum (P < 0.01). Compared with the model group

the treatment groups significantly improved liver fiber interval and degeneration degree.The results of immunohistochemistry showed that the contents of Collagen Ⅰ

Collagen Ⅲ and FN in liver cirrhosis model group and REF group were significantly higher than those in the normal group (P < 0.01).The contents of LN and Desmin in the model group wree significantly higher than those in the normal group (P < 0.01)

and the treatment group can significantly decreased integral absorbance (IA). Conclusion: The combination of REF and JF can obviously improve the pathological process of cirrhotic ascites in rats

increase the amount of urine

and promote the excretion of ascites.The mechanism may be related to the regulatory effect of REF combined with JF on Collagen Ⅰ

Collagen Ⅲ

FN

LN

Desmin factors.